Sakura Tissue-Tek Polar DM, Руководство по эксплуатации

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2-5
Preparing for Sectioning
Preparing for Frozen Block Preparation
1. Put the tissue sample (specimen) in the Cryomold [1].
2. Put OCT compound by making sure it does not overflow [2]. Be careful not to let air bubbles enter.
3. Put the Object Holder on top [3].
Make sure the side bearing the number comes to the tab side of the Cryomold.
Freeze the specimen. Refer to “Freezing in the Chamber” below for how to freeze specimens.
The Cryomold and Object Holder used should have appropriate shapes.
If compound, etc., is attached to the installation surface or tapered part of the Object Holder or to the
specimen head, the Object Holder may not be installed properly and cause sectioning problems or
instrument malfunction. Install the Object Holder after removing any unnecessary deposits.
Freezing in the Chamber
In addition to the "Chamber Freezing Method" where specimens are frozen in the
chamber," methods to freeze specimens include the "Liquid Nitrogen Method," "Dry
Ice Acetone Method" and "PINO Hyfluid Method. "Select an appropriate preparation
method according to the purpose.
Refer to p. 2-37 for the "PINO Hyfluid Method."
1. Press the "Cryo+" button among the buttons on the right side of the screen to
start rapid cooling. Continue cooling until the Cryo+ temperature drops to -50
°
C
or below. Cooling will continue for 15 minutes after the button is pressed.
To stop cooling, press the "Cryo+" button again. Refer to p. 1-20 for the Cryo+.
2. Put the Cryomold on the Cryo+ in the chamber [1].
Make sure the Object Holder side is facing up.
3. Place on the Object Holder the heat extractor that has been cooled beforehand
on the parking plate set on the Cryo+ [2]. You can do this easily using the arm
found on the left side face inside the chamber.
The specimen freezes quickly as it is cooled from both above and below.
Refer to p. 2-35 for information on how to set the parking plate and cool the heat
extractor.
4. When the specimen has been frozen, hold the knob on the Object Holder,
separate the Cryomold, and set the specimen on the specimen head. Refer to p.
2-6 for the setting method.
When separating the Object Holder from the Cryomold after cooling, wear protective gears and be sure to keep any
contact with the Object Holder brief. You may get frostbite.
When a cooling spray is used, use a cooling spray containing HFC-134a gas (1,1,1,2-Tetrafluoroethane) as a main
component. Do not use a cooling spray containing butane or propane. Use of such sprays may cause instrument
malfunction
Cryo+ button
Cryo+
(4 locations) Unfrozen
block
* The side
bearing the
number should
face up.
Heat extractor