6 - Operation
Operating in Routine Operation Mode
Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00
6 - 15
6.3.4.3 Preparation of Samples
Visually inspect the samples before pipetting. They must be free of:
Clots
Foam
Droplets on the tube wall
For this purpose we strongly recommend that you centrifuge the samples before
pipetting. After the sample collection wait for at least 10 minutes before
centrifuging the sample.
Maximally fill the sample tubes to 80%.
The sample tubes must not contain any additional (non-conductive) inserts or
have covers.
When using monovettes with plunger, the plunger must first be retracted fully
and only then be broken off. This method ensures a good contact to the
worktable (liquid detection).
If pipetting from gel monovettes is intended, make sure you use only sample
tubes with a sufficient amount of supernatant.
Note:
For further information on sample preparation, please refer also to the
recommendations given by your manufacturer and by the WHO.
6.3.4.4 Connecting Liquid Containers
When connecting liquid containers, also pay attention to the maintenance
instructions given in section
Tubing from Pressure Relief Valve
If your instrument is equipped with FWO/SPO/MPO, pay attention to the following:
Note:
To minimize the contamination risk, Illumina recommends you to connect
the bypass tubing from the pressure relief valve to the waste container (not back
to the system liquid container).
ATTENTION
Liquid handling problems due to air in the liquid system
If you direct the bypass tubing from the pressure relief valve back to the
system liquid container, make sure that the bypass liquid flow does not cause
bubbles in the system liquid.
Separate the bypass tubing and the aspiration tube in such a way that no air
bubbles can be aspirated.
Fig. 6-4
Droplet on wall