4 - Description of Function
Function
Infinium LiHa and Infinium RoMa Operating Manual - Document # 1000000110155 v00
4 - 7
4.3.3 Barcode Scanner on RoMa
Although the Robotic Manipulator (RoMa) arm is traditionally used to transfer
labware (eg, microtiter plates, tip boxes) around the instrument worktable, this
function is not utilized for the Illumina Infinium assay or platform. Because of this,
the RoMa grippers are usually removed by the Illumina FSE prior to installing the
RoMa-mounted barcode scanner, which is used by customers who require
barcode tracking, as integrated into either Illumina or Clarity LIMS, or their own
custom LIMS system.
The Illumina 1D barcode scanner kit is installed on the RoMa arm for all such
customers. It is mounted as per ILMN FSB 506 on the bottom of the RoMa arm
using special mounting hardware and an adjustable dual bracket, then connected
electrically via its cable to the existing RoMa arm cable. The latter powers the
scanner and transmits all data back and forth between IAC and the scanner. After
performing the scanner alignment using various IAC tools (as outlined in
FSB 506), the RoMa-mounted barcode scanner is then automatically positioned
and used by the automated IAC method to scan all plate and beadchip barcode
labels on the worktable, or in the TeFlow, that are used within an Infinium run. By
adjusting the dual bracket to either Position A (30 degrees for all MSA workflows
except MSA7 and MSA9), or Position B (60 degrees for MSA7 and MSA9), the
RoMa barcode scanner can read all legacy and newer labware barcode labels by
virtue of the selected scanning angle, which is manually adjusted prior to starting
an automated Infinium run. As a result, DNA plates and their respective
downstream MSA plates and beadchips can be tracked via LIMS throughout the
entire three day assay. Please note that all reagent tube barcodes are scanned
during automated IAC runs using the POSID3 barcode scanner, which comes
standard on all new Infinium RoMa instruments.