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TM397 · Revised 12/16
j. Next select all wells that have the same dilution factor or concentration. Once the wells are selected,
touch the Concentration or Dilution text box and assign the concentration or dilution factor value to the
selected wells (Figure 52).
Note:
The
Clear
button can be applied if a mistake is made during template creation. Clearing a well
removes all well type, naming and concentration/dilution factor information associated with the well.
F
13909T
A
igure 52. Concentrations and dilution factors.
In this figure, concentrations have been applied to the
standard curve replicates (shown at the bottom of each well) and dilution factors have been applied to the
unknowns (100 for wells A1–D8, 200 for wells E1–H8). These different dilution factors will be denoted during
the Well Grouping step described in Step k.
k. After all well types, names and concentrations/dilution factors have been applied to the well map, select
all wells that define an experimental set that should all be analyzed together (Knowns, Unknowns and
Blanks) and touch the
Well Grouping
button to automatically define replicates within your plate map.
The well grouping function identifies wells with the same well type, name and concentration/dilution
factor and automatically groups them into a replicate set and adds a replicate number to the upper right
corner of the well (Figure 53).
