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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM397 · Revised 12/16
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3.C. Protocol Screen (continued)
5.
Rearrange the steps by touching and dragging the appropriate steps.
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Figure 32. Loop function with detection modes selected.
Kinetics Function
The
Kinetics
function is used to perform a set of steps on a
per-well basis
, then repeating the set of steps a
desired number of times (i.e., inject and read one well multiple times before proceeding to the next well).
Note:
Kinetics can be used with all detection modes, plate types, and protocol functions except for heat, shake and
user prompt functions. If shaking and/or heating is desired in a kinetic protocol, they must be applied to the entire
plate and therefore be placed outside of the kinetic step. Loops may not be placed within a kinetic step, but kinetic
steps may be placed within Loops. A maximum of 250 kinetic reads in 1-unit increments can be performed within
a single protocol. For example, 10 readings with a 1-second interval means that 1 reading is taken every 1 second
for 10 seconds.
1.
Add the
Kinetics
step to the protocol by dragging and dropping the
Kinetics
icon onto the protocol.
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Figure 33. Kinetics function.
2.
All well-associated operations within the kinetic block must have the same well selection. The plate maps of
these operations are not individually editable.
