Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
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TM397 · Revised 12/16
11634T
A
Plate Map is
locked.
Select Plate Map.
Apply same
Plate Map
for each
protocol step.
Figure 20. When the plate map is locked, the map is applied to each step of the protocol.
When the plate map is unlocked, each step of the protocol can have a different plate map. The plate map in the
upper right corner in this case is shaded and cannot be modified, whereas the plate maps within each step of the
protocol are now active and the user can modify each step as desired.
F
11635T
A
Plate Map is
unlocked and
cannot be
applied.
Select Plate Map
for each
protocol step.
igure 21. When the plate map is unlocked, a different plate map can be created for each step.
Whenever a protocol is locked, the plate map that is indicated in the upper right corner will apply to each protocol
step. Notice in Figure 21, step 1 of the protocol has columns 1–3 selected, and step 2 has columns 9–12 selected.
However, the global plate map has columns 1–6 selected. When locking the protocol, each step will revert to the
global plate map (columns 1–6) or to whatever new plate map is selected.
Luminescence and Fluorescence Integration Times
Integration times for luminescence reads may be selected from 0.1 to 10 seconds.
Fluorescence reads may select
High Speed
, which uses a 0.1 second integration time, or
High Sensitivity
,
which uses a 0.3 second integration time.