Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
33
www.promega.com
TM397 · Revised 12/16
2.
To view results in real-time as a protocol is running, the plate map will automatically appear on the screen,
and the data will appear as they are collected. If more than one measurement is taken, each result may be
displayed by selecting the step that generated that result in the left navigation column.
Results are shown in a heat map format to indicate wells with higher or lower signals in comparison
to the entire plate. After all data are collected, the software automatically scales the color selection,
assigning the highest values red and the lowest values blue, along a gradient.
11
834M
A
7.56e+07 1.02e+07 1.03e+06 9.82e+04 1.31e+04 3.22e+03
298
72
Figure 36. Example GloMax
®
Discover Results heat map colors.
Within the plate map, each individual sample may be viewed by tapping the well of interest. If a Ratio-
metric protocol was performed the ratio of the Acceptor/Donor is also calculated and displayed.
For Absorbance measurements, both the Reference wavelength value and Experimental wavelength
value are reported. Reference values are subtracted from the Experimental values, and this calculation
is also reported in the result file.
Kinetic results display a graphical representation of the data during the course of the kinetics read. Data
are automatically scaled based on the results within each well. Zoom in to view the axis for each well as
desired. Users can use touch-screen gestures to zoom in or out, or to pan the well map.
11
652T
A
Turn the heat
map on or off.
Select
measurement 1
(e.g., Lumi) or
measurement 2
(e.g., Fluo) to
view results.
Figure 37. GloMax
®
Discover ‘Results’ screen with heat map display.
