User
Guide
for
Axoclamp
900A
Power
Supply............................................................................................................................... 21
Supply
Voltage
Selection ........................................................................................................ 21
Changing
the
Fuse ................................................................................................................... 22
Glitches...................................................................................................................................... 22
Grounding
and
Hum .................................................................................................................. 23
Chapter
3:
Setup
and
Tutorials..................................................................................................... 25
Checklist........................................................................................................................................ 25
Axoclamp
900A
and
Axoclamp
900A
Commander
Setup..................................................... 26
Tutorial
1:
Current
Clamp
(IC) .................................................................................................. 28
Tutorial
2:
Discontinuous
Current
Clamp
(DCC) ................................................................... 34
Tutorial
3:
Discontinuous
Single
‐
Electrode
Voltage
Clamp
(dSEVC).................................. 37
Tutorial
4:
Two
‐
Electrode
Voltage
Clamp
(TEVC) ................................................................. 41
I.
TEVC
Using
the
CLAMP
‐
1U
Model
Cell.......................................................................... 41
II.
TEVC
Using
the
MCO
‐
2U
Oocyte
Model
Cell
and
VG
‐
9A
Bath
Clamp ..................... 46
DC
Restore................................................................................................................................ 49
Tutorial
5:
Auto
Mode
Switching .............................................................................................. 50
Example
of
Auto
Mode
Switching ........................................................................................ 51
Setup
of
Auto
Mode ................................................................................................................ 52
Tutorial
6:
High
‐
Voltage
Current
Clamp
(HVIC) ................................................................... 55
Chapter
4:
Guide
to
Electrophysiological
Recording............................................................... 59
General
Advice............................................................................................................................. 59
Chamber
Design ...................................................................................................................... 59
Perfusion ................................................................................................................................... 60
Mechanical
Stability ................................................................................................................ 60
Optics......................................................................................................................................... 61
Bath
Electrode .......................................................................................................................... 61
Interfacing
a
Computer........................................................................................................... 62
Noise.............................................................................................................................................. 62
Measurement
of
Noise ............................................................................................................ 62
Sources
of
Noise....................................................................................................................... 63
Bath
Error
Potentials ................................................................................................................... 65
(1)
Minimize
R
b
......................................................................................................................... 66
(2)
Clamp
V
Using
a
Bath
Clamp
or
Virtual
Ground
b
........................................................ 67
(3)
Independently
Measure
V
and
Subtract
it
from
the
CHANNEL
1
Potential
b
........... 68
Summary................................................................................................................................... 70
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