User
Guide
for
Axoclamp
900A
ο
VG
‐
9A
×100
(bath
clamp
head
stage).
Two
model
cells:
ο
CLAMP
‐
1U.
ο
MCO
‐
2U
(oocyte).
Note
that
most
of
the
tutorials
use
the
HS
‐
9A
×0.1
and
HS
‐
9A
×1
headstages
and
the
CLAMP
‐
1U
model
cell
that
come
standard
with
the
Axoclamp
900A.
However,
one
of
the
TEVC
tutorials
requires
a
different
set
of
headstages,
HS
‐
9A
×10
and
VG
‐
9A
×100,
and
a
different
model
cell,
the
MCO
‐
2U.
If
you
don’t
have
the
specific
headstages
or
model
cell
called
for
in
the
tutorial,
you
can
substitute
your
headstages
in
the
tutorials.
But
be
aware
that
some
of
the
scaling
will
be
different.
Axoclamp
900A
and
Axoclamp
900A
Commander
Setup
We
recommend
performing
the
initial
setup
and
tutorials
using
the
Axoclamp
900A,
the
associated
computer
and
an
oscilloscope
in
a
standalone
fashion
on
a
bench
top.
The
tutorials
may
also
be
performed
at
a
later
date
with
the
equipment
mounted
in
a
typical
laboratory
setting.
1.
Connect
the
Axoclamp
900A
to
power
source
with
power
cable.
2.
Connect
a
USB
cable
between
the
computer
and
the
CONTROL
USB
port
at
the
back
of
the
Axoclamp
900A.
3.
Connect
another
USB
cable
between
the
computer
and
the
SCOPE
USB
port
at
the
back
of
the
Axoclamp
900A.
4.
Install
the
Axoclamp
900A
Commander
software
on
the
computer
to
control
the
Axoclamp
900A.
At
the
Hardware
Installation
message
about
the
Universal
Service
Bus
controllers,
click
Continue
Anyway
to
allow
installation
to
proceed
to
completion.
There
is
no
need
to
turn
off
the
power
to
the
Axoclamp
900A
prior
to
connecting
headstages.
However,
do
not
disconnect
headstages
while
the
software
is
starting
up.
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