Installation
and
Basic
Operation
function
of
each
of
the
buttons,
as
well
as
the
keyboard
shortcut
for
that
function.
The
Options
tool
button
opens
a
dialog
box
where
many
basic
Axoclamp
900A
Commander
parameters
are
set.
Meters
The
Meters
at
the
top
of
the
Axoclamp
900A
Commander
main
window
are
grouped
into
two
headstages
(unless
you
have
one
headstage
only,
in
which
case
only
one
headstage
appears).
By
default,
each
channel
displays
the
potential
in
millivolts
(mV)
and
the
current
in
picoamps
(pA)
or
nanoamps
(nA).
Total
Resistance,
Rt
Check
the
Total
Resistance
(Rt)
checkbox
to
display
resistance
in
M
Ω
on
the
top
meter
for
either
channel.
This
can
be
activated
in
either
voltage
‐
clamp
or
current
‐
clamp
modes,
but
not
I=0
mode
or
with
slow
current
injection.
Note
that
to
measure
resistance
the
Axoclamp
900A
Commander
generates
a
command
signal,
thus
the
current
reading
fluctuates
markedly
when
resistance
measurement
is
enabled.
Mode
Buttons
The
Mode
buttons
set
the
amplifier
mode—current
clamp
at
zero
(I=0)
or
current
clamp
(IC)
for
each
of
the
two
headstages:
Headstage
1
supports
discontinuous
current
clamp
mode
(DCC)
and
discontinuous
single
‐
electrode
voltage
clamp
mode
(dSEVC).
Headstage
2
supports
high
‐
voltage
current
clamp
(HVIC)
and
two
‐
electrode
voltage
clamp
mode
(TEVC)
when
used
in
conjunction
with
headstage
1.
Click
on
a
mode
button
to
switch
to
the
selected
mode.
The
change
is
confirmed
by
the
indicator
lights
on
the
front
panel
of
the
Axoclamp
900.
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