User
Guide
for
Axoclamp
900A
HVIC
(Channel
2)
Internal
holding
level
max:
±0.13–13.0
μ
A
(depends
on
headstage).
External
command
sensitivity:
10,
100,
or
1000
nA/V
(depends
on
headstage).
dSEVC
(Channel
1)
Internal
holding
level:
±200
mV.
AC
voltage
‐
clamp
gain:
0.003–30
nA/mV,
0.03–300
nA/mV,
0.3–3000
nA/mV
(depends
on
headstage).
External
command
sensitivity:
20
mV/V.
Voltage
rise
time:
250
μ
s.
Current
settling
time
2
:
500
μ
s
to
10%
of
peak
value.
Voltage
noise
2
:
180
μ
V
rms
.
Current
noise
2
:
0.30
nA
rms
.
TEVC
(Channels
1
&
2
together)
Internal
holding
level:
±200
mV.
AC
voltage
‐
clamp
gain:
20
to
50,000.
Voltage
‐
clamp
lag:
5.4
μ
s–52
ms.
2
Model
cell
with
two
50
M
Ω
resistors
to
simulate
the
electrode
resistances
and
a
50
M
Ω
resistor
and
470
pF
capacitor
in
parallel
to
simulate
the
cell
membrane.
10
mV
step.
Lowpass
filter,
10
kHz.
Voltage
‐
clamp
Gain,
2.2
nA/mV;
Lag,
minimum.
Sample
rate,
20
kHz.
Pipette
Capacitance
Neutralization,
1.5
pF.
Set
for
flattest
membrane
step
response.
HS
‐
9A
×0.1
headstage.
176
