LC Packings Probot 161403, User Manual

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Appendix D
User’s Manual Probot
D960R3
D-9
Trigger PeakEnd UV_VIS_1 < 5 and UV_VIS_1.Delta > -
0.03, True=2.00
Pump_Relay_2.State = On
Pump_Relay_2.Duration = 1.00
EndTrigger
30.000 %B
= 50
31.000 %B
= 90
36.000 %B = 90
36.100 %B = 0
55.000 UV_VIS_1.AcqOff
PumpPressure.AcqOff
ColumnPressure.AcqOff
Pump.Flow
= 4.000
%B = 0
%C = 0
%D = 0
End
D.6 Additional Hints/Troubleshooting
For the proper handling of a fractionation task, it is important to calculate or
measure the delay time between the flow cell of the detector (where the peak is
detected) and the fractionation needle (where the peak then will be collected).
The Probot can be started and will wait for a start signal before the first peak if
the ‘Wait for contact start signal’ option is enabled on the Relay Table tab of the
Fractionate task (FIGURE D-7). In addition, you need to connect the START input
of the Probot to the start output of the starting device using the Start Cable (see
also Section B.3.
If two Peak Start (TTL 1) signals are generated immediately after each other, the
Probot moves to the next vial.
If two Peak End (TTL 2) signals are generated immediately after each other,
nothing will happen.
If two signals (TTL1 or TTL2) are generated very shortly after each other, the
Probot may home itself and after that continues the run.
For additional information refer to the µCarrier user’s manual (e.g. Fractionation
Task), the CHROMELEON on-line help (e.g. tutorial, see trigger) and to the
UltiMate User’s Manual.