LC Packings Probot 161403, User Manual

Page: 33 / 118

Installation and Getting Started
User’s Manual Probot
D960R3
2-13
2.6.2 Design of µCarrier
The µ Carrier Liquid Handling Application Designer Software (V2.0) is built around
the following three basic components:
•
The sample carrier – represents the target (e.g. a MALDI target or a microtiter
plate), which is used. It describes the number of spots on this target and their
position relative to each other.
•
The tray – represents a number of sample carriers and there positions on the
table. It is required to define the position of every spot on the tray. By aligning
the sample carriers, every spot on the MALDI targets is defined with respect to
the exact X, Y and Z coordinate relative to the needle of the Probot.
• The application – represents all ‘rules’ (tasks) required for spotting. A
collection of spots is grouped and these groups are used within the different
tasks. Every spot is defined by its X, Y and Z coordinates, so each spot can be
found individually. This allows you to use your MALDI targets in any way you
want (e.g. you can collect two runs on one MALDI target or one run on two
MALDI targets). The application file holds the parameters for the sample
carriers, the tray and the tasks.
For each component a special wizard assisted ‘Editor’ is available.
2.6.3 The Editors and Wizards
For the ease of use µCarrier provides various wizards and editors for the design
of the sample carrier, the tray and the application:
• Sample Carrier Alignment Wizard – Section 2.7.3
• Sample Carrier Wizard – Section 2.8.1
• Sample Carrier Editor – Section- 2.8.1
•
Tray Setup Wizard – Section 2.8.2
• Tray Editor – Section 2.8.3
•
Fractionation Application Wizard - Section 2.8.4
• Application Editor – Section 2.8.4
Depending on the editor which is currently active or the task that is to be done
next, the corresponding wizard starts automatically if the New button is selected.
2.6.4 Necessary Steps to create and run the Application
A series of basic steps is required to create and to prepare and to run your
application. For the ease of use, µCarrier V2.0 provides predefined sample
carriers and tray definitions for the most commonly used MALDI mass
spectrometer. In addition, predefined standard applications are available to get
easily started.
Section 2.7.2 shows how to define applications using these definitions. Sections
2.8.1 - 2.8.4 show how to use the wizards to create new sample carriers, tray
and application.