LC Packings Probot 161403, User Manual

Page: 106 / 118

Appendix D
D-4
D960R3
User’s Manual Probot
D.3.3 Installing the Fused Silica Capillary
To install and adjust the fused silica capillary properly in the needle tee, follow
the instructions below:
a) Depending on your application (e.g. capillary or Nano LC) use either the
30
µ
m I.D. fused silica capillary (P/N 162144, for Nano LC) or the 100
µ
m
I.D. capillary (P/N 162145, for capillary LC) and one Teflon connector from
the accessory kit to extend the outlet capillary of the flow cell.
b) Install the fused silica capillary into the metal port above the needle tee using
the installed nut, a ferrule and a PEEK sleeve; then guide the capillary
through the upper port the needle tee and all the way through the needle
(FIGURE D-4).
1
FIGURE D-4 Installing the Fused Silica Capillary
c) Turn the fitting until it is finger tight. You should not be able to move the
capillary laterally along the tubing axis (item 1, FIGURE D-4).
d) Pre-adjust the fused silica capillary inside the stainless steel needle so that
the end of the stainless steel needle extends the fused silica capillary by
approximately 1 mm.
e) Start the µCarrier Software and control the proper adjustment of the fused
silica capillary and the table (Section D.4.2).