LC Packings Probot 161403, User Manual

Page: 39 / 118

Installation and Getting Started
User’s Manual Probot
D960R3
2-19
2.7.3 Alignment of the Sample Carriers
The following procedure needs to be performed to define the exact position of
the sample carriers on the table relative to the needle tip (including the ‘spotting
height’). During this procedure the table is moved manually to certain reference
positions and their coordinates (X, Y, Z) will be stored in the
µ Carrier Software.
Once this procedure has been performed successfully, this setup can be used as
long as the hardware has not been modified (e.g. the needle has been replaced or
bent).
Note: The proper alignment is essential to achieve optimal spotting performance.
Once the hardware has been modified (e.g. the needle has been replaced or bent
or a different table is used) or the Probot has been moved, it is necessary to
repeat this procedure.
If the tray definition includes the exact positions of all sample carriers relative to
each other (e.g. multiple sampler carriers of the same type in predefined
positions), the µCarrier software needs only one spot as reference position to
align all carriers in one step. If the tray definition does not provide the exact
(relative) X and Y positions, the alignment has to be performed for each sample
carrier individually.
To align the sample carriers:
a) Open the Sample Carrier Alignment Wizard by selecting Sample carrier
alignment from the Tools menu. The introduction window (FIGURE 2-14)
presents the origin of reference sample carrier as red diagonal cross.
Red ‘X’ represents the
origin of the reference
sample carrier.
Current coordinates.
Z = Spotting Height
FIGURE 2-14 Introduction Screen of the Sample Carrier Alignment Wizard
b) To select one or more carriers, draw a frame around the origins (the red ‘+’)
of all sample carriers while keeping the left mouse button pressed. After the
selection the origin of the reference carrier is presented by a red ‘X’
(FIGURE 2-14).