LC Packings Probot 161403, User Manual

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Appendix D
D-6
D960R3
User’s Manual Probot
c) Select the NeedleDown button and that the stainless steel needle covers the
tip of the fused silica capillary (FIGURE D-1).
d) Calibrate all sample carriers (position and height) with the stainless steel
needle in the down position (e.g. the fused silica capillary is not visible).
Make certain the needle is just above the sample carrier and does not touch
the carrier.
e) Set the sample carrier to the calibrated height, and then put the (stainless
steel) needle up. Make certain that the distance tip of the fused silica
capillary and the carrier is between 1 and 2 mm (0.004 - 0.008 inch).
D.4.3 Using the Waste Removal Option - Example
The following example shows how to use the waste removal option in
conjunction with the peak detection capabilities of CHROMELEON.
To setup the µCarrier software:
a) Prepare the tray in the standard way; define as many groups as required for
the runs. Because of the waste removal option a waste group is not required.
b) Add an ‘Advanced Fractionate’ task and adjust the General parameters as
appropriate for your application.
FIGURE D-7 General tab of the Fractionate Task
c) Fill in the correct ‘Flow …’ and the ‘Dead volume…’.
d) Disable the ‘Reagent addition’ option.