horiba ABX MICROS ES60, User Manual

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ABX Micros ES60 OT/CT
7–32 User Manual - RAB237AEN
◆
Once the CV%s have been calculated, compare them to the specified limits.
5.2.4. Calibration
◆
If the system seems to be operating properly, fresh uncontaminated reagents are being used and
the precision is within the specifications but the problem still occurs, the ABX Micros
ES60 OT/CT
may need calibration (See Section 5, 4. Calibration, page 5-21 ).
5.3. Troubleshooting parameters
◆
The procedures described below should be performed whenever the precision of a parameter is not
within its CV specifications or the parameter is incorrect or suspicious.
◆
When all parameters are affected, it is necessary to look for a common cause (e.g. vacuum,
pressure, sample aspiration, chamber filling and draining correctly, common reagent pollution,
etc....).
◆
When only one parameter is affected, it is necessary to look for a cause in this specific area of
analysis. The different tables listed, give the noted problems for the parameter in question,
possible cause of the problem and the necessary user action to correct the problem.
5.3.1. Problems on all parameters
Tab.7-5 Problems on all parameters
Diag.7-40 Calculation factors for CV% determination
Definitions:
◆
X = the calculated Mean
◆
n = the total number of samples
◆
Xi = the individual parameter value
◆
SD = Standard Deviation
=> CV% = (SDx100)/X
Problem noted Possible cause Corrective action
Startup failed
Out of reagent(s)
Check and/or replace reagent(s) if necessary
(See
3.5. Reagent installation, page 7-13 )
Contaminated reagent(s)
Check and/or replace reagent(s) if necessary
(See
3.5. Reagent installation, page 7-13 )
Possible dirty apertures
Perform a concentrated cleaning (See 3.3.5.
Concentrated cleaning, page 7-9 ), rerun
startup
No results in analysis
Blocked sampling needle
Remove and flush out sampling needle (See
3.7. Unblocking sampling needle, page 7-17 )
Sampling needle depth not
adjusted correctly
Possible defective Pre-amp on
main board
Contact your local HORIBA ABX Technical
Support Representative for assistance
Suspicious patient
results
Possible dirty apertures
Perform a concentrated cleaning (See 3.3.5.
Concentrated cleaning, page 7-9 ), rerun
sample
Possible partial blocked sampling
needle Remove and flush out sampling needle (See
3.7. Unblocking sampling needle, page 7-17 )
Possible leakage from syringe
blocks
Check syringe block for leakage and contact
your local HORIBA ABX Technical Support
Representative
Possible fibrin clot in "T" fitting
below WBC chamber possible tube
cap debris in "T" fitting below
WBC chamber
Contact your local HORIBA ABX Technical
Support Representative
X
xi
n
----
∑
=
SD
X Xi
–
〈 〉
2
∑
n 1
–
------------------------------ =