horiba ABX MICROS ES60, User Manual

Page: 64 / 200

ABX Micros ES60 OT/CT
3–8 User Manual - RAB237AEN
Results
◆
A certain amount of cells will pass through the calibrated micro-aperture within a specific time
frame. They are then measured by pulse height, thresholded, grouped by size and mathematically
calculated along with the calibration coefficient to give a final numerical value for both RBCs and
PLTs.
RBC and PLT histograms
◆
The RBC and PLT histograms are determined by thresholding of the electronical pulses. These
pulses are then grouped according to size by channeling the pulses into the correct size category.
The electronic pulses are smoothed mathematically and plotted on a graph.
◆
RBC histogram: is an electronic distribution and mathematical calculation of the RBCs placed into
256 channels of volumetric sizing from 30fl to 300fl.
◆
PLT histogram: is an electronic distribution and mathematical calculation of the PLTs placed into
128 channels of volumetric sizing from 2fl to a mobile threshold between the high end platelets
and the low end red blood cell thresholds.
(fl = femtoliters) A microscopic volumetric unit of measurement. This is a 3-dimentional
measurement used to determine the volume of microscopic particles.
Diag.3-10 Cells passsing through the aperture creating electronic pulses
Diag.3-11 RBC Pulses grouped according to the number and the size of the cells
Diag.3-12 RBC Pulses electronically calculated and smoothed: RBC distribution curve
Diag.3-13 PLT pulses electronically calculated and smoothed: PLT distribution curve
PLT pulses
RBC pulses
Cell size
Number of cells
Cell size
Number of cells
Cell size
Number of cells