horiba ABX MICROS ES60, User Manual

Page: 62 / 200

ABX Micros ES60 OT/CT
3–6 User Manual - RAB237AEN
Tab.3-3 HGB measurement
◆
10µl of blood sample contained in the sampling needle is pushed with 1.2ml of ABX Minidil LMG
along with 0.5ml of ABX Minidil LMG from the outer needle into the WBC/HGB chamber.
◆
0.4ml of ABX Minidil LMG is added into the WBC/HGB chamber making the diluent volume 2.1ml.
◆
0.5ml of ABX Lyse is added to the mixture.
◆
This makes the total dilution for WBC/HGB.
3.2.2. RBC/PLT
Tab.3-4 RBC/PLT dilution
Tab.3-5 RBC/PLT measurement
◆
28.3µl of diluted blood is aspirated from the WBC chamber. The needle carriage assembly moves
to the right over the RBC/PLT chamber.
◆
2.0ml of ABX Minidil LMG and the 28.3µl of diluted blood is injected into the RBC/PLT chamber
from the inner sample needle.
◆
0.5ml of ABX Minidil LMG is added to the dilution in the chamber from the outer sample needle.
This makes the total dilution for the RBC/PLT.
HGB measurement
Method Photometry
Wavelength 550nm
Count period 2x5 seconds
Temperature reaction Ambient
RBC/PLT dilution
Initial diluted blood volume 28.3µl: rate 1/170
Volume of ABX Diluent 2.5ml
Final dilution ratio 1/15 000
RBC/PLT measurement
Method Impedance
Aperture diameter 50µm
Count Vacuum 200mb
Count period 2 (or 3) x 6 seconds
Temperature reaction Ambient