ABX Micros
ES60
OT/CT
5–28
User Manual - RAB237AEN
4.6. RDW calibration
Introduction
◆
The RDW calibration is a separate calibration outside the calibration menu.
◆
The RDW calibration coefficient default value is normally set at
1.00
.
RDW calibration using a quality control standard
◆
The RDW may also be calibrated by using a known quality control standard.
1-
Take the controls and bring them to room temperature. Gently and thoroughly mix the control
material as indicated on the instruction sheet that comes with the control kit.
2-
Run the normal level control 6 times, as a regular patient analysis. When the cycles are complete,
note only the RDW results. Write down these results for future reference use.
3-
Calculate the mean value for all 6 results and write it down for future reference use.
4-
Take the control assay sheet that comes with the control kit and note only, the normal control
mean assay value for RDW.
5-
Calculate the new RDW coefficient as follows:
Take the normal control mean assay value for RDW. Divide it by the mean value of the normal
control ran 6 times as a sample, times the current RDW calibration coefficient. This will equal the
new RDW calibration coefficient.
6-
To enter this new value See
4.7.1. Changing calibration coefficients, page 5-29
7-
Now take all 3 levels of controls (low, normal, high) and run them 1 time each as a regular patient
analysis. When the cycles are complete, note only the RDW results and compare them to the control
assay values for RDW, on the assay sheet. Verify that the RDW results are somewhat close to the
mean assay values and within the ranges as specified for all 3 Levels.
8-
The RDW calibration is now complete. Be sure to monitor your RDW results and verify that they
fall within your patient population.
9-
Verify that normal RDW results will be within the established ranges set in the settings menu. See
6.3. Analysis limits, page 6-12
.
The RDW calibration is normally performed by taking blood samples from 100
healthy, normal and drug free individuals. These blood samples are then
analyzed on the instrument that has been calibrated for RDW determination.
The mean and standard deviation are then calculated from that population
analyzed. The same 100 samples are then analyzed on the ABX Micros
ES60
OT/CT. A population mean is calculated and then compared to the known
calculated mean from the comparison instrument. The RDW calibration
coefficient for the ABX Micros
ES60
OT/CT is then calculated from the
difference of the two mean values.
Expected RDW values may vary with sample population and/or geographical
location. It is highly recommended that each laboratory establish its own
normal ranges based on the local population.
DEFINITION: A quality control standard is defined as a commercial blood
product which has been specifically developed and assayed with set parameter
target values and ranges. This product is designed to measure precisely the
accuracy and linearity of the analyser.
Certain pathological conditions will affect the RDW results. See Section 2,
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