Bio-Tek ELx405, Operator'S Manual, Page: 191 / 250

Liquid Tests
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161
If the %CV is greater than 4.0, one or more dispense tubes may need to
be cleaned. Run AUTOCLEAN (Ultrasonic Advantage™ models only),
and/or remove the manifold and use the stylus to clean the dispense
tube(s) giving lower-than-average absorbance readings (all models).
When finished, re-prime the washer and retry the test.
8. When finished, prime with deionized water to flush out the dye solution.
With the Valve Module
Routine Procedure for Valve A
Perform this test during the initial/annual OQ , and the monthly
PQ .
1. Fill the supply bottle for Valve A with two liters of deionized water. Run
the Maintenance program DAY_RINSE two or three times to prime the
fluid lines and the manifold.
2. Fill the supply bottle for Valve A with 1440 ml of Solution #4 .
3. Run DAY_RINSE to prime Valve A with the solution.
4. Place a clean, dry 384-well microplate on the washer and run the Dispense
program
192accuracy_qc . This program dispenses 80 µ l of solution to
each well of the plate. It does not evacuate the solution.
5. When the program is finished, carefully remove the plate.
Note: If you are using one of Bio-Tek’s keypad-based readers,
such as the ELx800NB  , ensure that the reader is not running in
Rapid Mode . To check the setting, select
UTIL READ
and cycle
through the options until
READ IN RAPID MODE?
appears. Set it to
NO .
6. Read the plate in an optical reader (blank on air), using the
dual-wavelength method (630 nm - 450 nm), then print or export the
results.
7.
Using the
Dispense Precision Test Worksheet
on page 169, perform data
reduction:
a. Calculate the
Standard Deviation .
Tip: If you have a spreadsheet software program, enter/export all 384
values into a spreadsheet and apply your program’s Standard
Deviation function (e.g., Microsoft Excel’s STDEV).
b. Calculate the sum of the OD values for all 384 wells, then divide by
384
to determine the Mean OD for the plate.
ELx405 Operator's Guide