Bio-Tek ELx405, Operator'S Manual

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Liquid Tests
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155
If the %CV is greater than 4.0, one or more dispense tubes may need to
be cleaned. Run the AUTOCLEAN (Ultrasonic Advantage™ models
only), and/or remove the manifold and use the stylus to clean the
dispense tube(s) giving lower-than-average absorbance readings (all
models). When finished, re-prime the washer and retry the test.
8. When finished, prime with deionized water to flush out the dye solution.
Annual Procedure for All Valves
Perform this test during the annual OQ only.
The routine test for Valve A (see previous page) must pass before the
annual test for Valves A-D can be performed.
1. Empty the waste bottle now, and then as needed throughout this
procedure.
2. Fill each of the supply bottles connected to Valves A, B, C , and D with
three liters (3000 ml) of deionized water. Place the bottles on a surface level
with the washer, i.e., the adjacent (lab bench) area.
Repeat the following steps for each valve:
3. Run the Maintenance program DAY_RINSE two or three times to prime
the fluid lines, manifold, and the valve being tested.
ELx405 standard models (Single/96 manifold): See the note on the
next page before performing step #4.
4. Edit the Dispense program accuracy_qc_test to use the Valve currently
being tested (
DEFINE EDIT DISP
accuracy_qc_test ).
5. Place a clean, dry 96-well microplate on the balance and zero the balance.
6. Place the microplate on the carrier and run the Dispense program
accuracy_qc_test . This program dispenses 300 µ l of water to each well of
the plate. It does not evacuate the wells.
7. When the program is finished, carefully remove the plate and weigh it. This
is the
Total Dispense Weight in grams.
The weight should be 28.8 grams, ± 10% (between 25.92 g and
31.68 g).
If the weight falls outside this range, contact Bio-Tek.
ELx405 Operator's Guide