Bio-Tek ELx405, Operator'S Manual

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154
| Chapter 5: Performance Verification/Qualification Tests
If the %CV is greater than 4.0, one or more dispense tubes may need to
be cleaned. Run
AUTOCLEAN
(Ultrasonic Advantage™ models only),
and/or remove the manifold and use the stylus to clean the dispense
tube(s) giving lower-than-average absorbance readings (all models).
When finished, re-prime the washer and retry the test.
8. When finished, prime with deionized water to flush out the dye solution.
With the Valve Module
Routine Procedure for Valve A
Perform this test during the initial/annual OQ , and the monthly PQ .
1. Fill the supply bottle for Valve A with two liters of deionized water. Run
the Maintenance program DAY_RINSE two or three times to prime the
fluid lines and the manifold.
2. Fill the supply bottle for Valve A with 1200 ml of Solution #3 .
3. Run DAY_RINSE to prime Valve A with the solution.
4. Place a clean, dry 96-well microplate on the washer and run the Dispense
program
accuracy_qc_test . This program dispenses 300 µ l of solution to
each well of the plate. It does not evacuate the solution.
5. When the program is finished, carefully remove the plate.
If you are using one of Bio-Tek’s keypad-based readers, such as the
ELx800
 or ELx808  , ensure that the reader is not running in Rapid
mode. To check the setting, select
UTIL READ
and cycle through the
options until
READ IN RAPID MODE?
appears. Set it to NO .
6. Read the plate in an optical reader (blank on air), using the dual-
wavelength method (630 nm – 450 nm), then print or export the results.
7. Using the
Dispense Precision Test Worksheet
on page 166, perform data
reduction:
a. Calculate the
Standard Deviation .
Tip: If you have a spreadsheet program, enter/export all 96 values into
a spreadsheet and apply your program’s Standard Deviation function
(e.g., Microsoft Excel’s STDEV).
b. Calculate the sum of the OD values for all 96 wells, then divide by 96 to
determine the Mean OD for the plate.
c. Calculate the %CV : (Standard Deviation / Mean OD) * 100.
The %CV should be <=4.0 .
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