Bio-Tek ELx405, Operator'S Manual

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| Chapter 5: Performance Verification/Qualification Tests
Dispense Precision Test (192)
This test is designed for the Dual/192 manifold. See page 153 if
you’re looking for the Dispense Precision Test for Single/96 and
Dual/96 manifolds.
See page 148 for a list of required materials.
Without Valve Module
1. Fill a supply bottle with two liters of deionized water. Run the Prime
program
P_DAY_RINSE two or three times to prime the fluid lines and
manifold.
2. Fill a supply bottle with 1440 ml of Solution #4 .
3.
Run
P_DAY_RINSE
to prime the washer with the solution.
4. Place a clean, dry 384-well microplate on the washer and run the Dispense
program
192accuracy_qc . This program dispenses 80
µ
l of solution to
each well of the plate. It does not evacuate the solution.
5. When the program is finished, carefully remove the plate.
Note: If you are using one of Bio-Tek’s keypad-based readers,
such as the ELx800NB  , ensure that the reader is not running in
Rapid Mode . To check the setting, select
UTIL READ
and cycle
through the options until
READ IN RAPID MODE?
appears. Set it to
NO .
6. Read the plate in an optical reader (blank on air), using the
dual-wavelength method (630 nm - 450 nm), then print or export the
results.
7. Using the appropriate
Dispense Precision Test Worksheet
on page 168,
perform data reduction:
a. Calculate the
Standard Deviation
.
Tip: If you have a spreadsheet software program, enter/export all 384
values into a spreadsheet and apply your program’s Standard
Deviation function (e.g., Microsoft Excel’s STDEV).
b. Calculate the sum of the OD values for all 384 wells, then divide by 384
to determine the Mean OD for the plate.
c. Calculate the
%CV
: (Standard Deviation / Mean OD) * 100.
The %CV should be <= 4.0 .
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