Bio-Tek ELx405, Operator'S Manual

Page: 187 / 250

Liquid Tests
|
157
Evacuation Efficiency Test (192)
This test is designed for the Dual/192 manifold. See page 150 if you’re
looking for the Evacuation Efficiency Test for Single/96 and Dual/96
manifolds.
See page 148 for a list of required materials.
1. Fill a supply bottle with two liters of deionized water. Run the Maintenance
program
DAY_RINSE two or three times to prime the fluid lines and
manifold.
2. Place a clean, dry 384-well microplate on the balance and zero the balance.
3. Pipette or dispense 80 µ l of Solution #1 into each well of the microplate.
4. Place the plate on the washer and run the Aspirate program
192
resid_qc_test . This program evacuates all of the wells, leaving a
small amount of residual fluid.
5. When the program is finished, remove the plate and weigh it immediately,
because evaporation will affect the results. This is the
Total Residual
Weight
,
in grams.
6. Visually inspect the plate and note if any wells appear to have considerably
more liquid in them than others.
7. Using the
Evacuation Efficiency Test Worksheet
on page 167, perform
initial data reduction:
a.
Divide the Total Residual Weight by
384
to find the
Mean Residual
Weight
.
b. The Mean Residual Weight should be
<=
0.002 g
.
If the Mean Residual Weight is greater than 0.002 g, or if one or more wells appear
to have much more liquid than the others, the washer failed the test.
Troubleshoot as follows:
•
If the test fails once: Failure of this test is most commonly caused by
improper aspiration tube placement within the well, usually because a
microplate other than the recommended Corning Costar
®
384 was used.
If you prefer to use a plate other than the Corning Costar 384, modifying
the ASPIRATE HEIGHT or HORIZONTAL ASPIRATE POSITION
parameters in a copy of the
192resid_qc_test
program should correct
this error. ( 192resid_qc_test is locked for editing, but you can copy the
program and edit its parameters in the newly created copy.) See
Chapter
3
,
Operation
, for instructions on copying and editing programs and
modifying the abovementioned parameters. After making this change,
retry the test using a clean, dry microplate.
ELx405 Operator's Guide