Bio-Tek ELx405 Operator'S Manual Download

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ELx405

™

Microplate Washer

Operator’s Guide

For all models:

ELx405
ELx405 Select
ELx405 Magna
ELx405 HT
ELx405 Select CW

Summary of Contents for ELx405

Page 1: ...ELx405 Microplate Washer Operator s Guide For all models ELx405 ELx405 Select ELx405 Magna ELx405 HT ELx405 Select CW...

Page 2: ......

Page 3: ...ELx405 Microplate Washer Operator s Guide January 2005 2005 7101000 Revision I Bio Tek Instruments Inc...

Page 4: ...ii Preface Bio Tek Instruments Inc...

Page 5: ...Stack and Ultrasonic Advantage are trademarks of Bio Tek Instruments Inc Microsoft Windows and the Windows logo are registered trademarks of Microsoft Corporation in the United States and other countr...

Page 6: ...iv Preface Bio Tek Instruments Inc...

Page 7: ...ing and Shipping xviii Warnings xix Hazards and Precautions xix Hazards xix Precautions xx Directive 89 336 EEC Electromagnetic Compatibility xxii Directive 73 23 EEC Low Voltage Safety xxii Directive...

Page 8: ...onal Accessories 9 Specifications 10 Performance Specifications 11 Technical Support 12 Phone Support 13 Electronic Communication 13 Facsimile Support 13 Written Communication 13 Shipping Address 13 A...

Page 9: ...ackaging the ELx405 and Its Accessories 39 Repackaging the External Valve Module if equipped 39 Chapter 3 Operation 41 ELx405 Front Panel 42 Keypad Description 43 Entering Program Names 44 System Star...

Page 10: ...pense Program 82 Aspiration 83 Run Aspiration Program 83 Define Aspiration Program 84 Edit Aspiration Program 84 Copy Aspiration Program 85 Delete Aspiration Program 85 Soak 86 Define Soak Program 86...

Page 11: ...ded Maintenance Schedule 103 Required Materials 104 Warnings Precautions 105 Maintenance Programs 106 Prime Programs 107 Rinse Soak the Fluid Path 109 Daily Maintenance 109 Overnight Multi Day Mainten...

Page 12: ...nse Precision Test Overview 146 Valve Module Test Overview 147 Which Tests to Perform 147 Materials 148 Evacuation Efficiency Test 96 150 Dispense Precision Test 96 153 Evacuation Efficiency Test 192...

Page 13: ...187 Fatal Errors 200 Bio Stack Errors 203 Appendix C Chemical Compatibility 205 Components and Materials Composition 206 Appendix D Default Programs 207 Default Programs 208 Appendix E Changing the M...

Page 14: ...hout External Valve Module 31 Figure 15 Washer With External Valve Module 32 Figure 16 Attaching the Mist Shield 33 Figure 17 ELx405 Front Panel With LCD and Keypad 42 Figure 18 Dispense and Aspirate...

Page 15: ...802 655 4740 outside the U S Fax 802 655 7941 E Mail customercare biotek com Service TAC Phone 800 242 4685 toll free in the U S 802 655 4740 outside the U S Fax 802 655 3399 E Mail tac biotek com Eur...

Page 16: ...ortant safety notes Warning A Warning indicates the potential for bodily harm and tells you how to avoid the problem Caution A Caution indicates potential damage to the instrument and tells you how to...

Page 17: ...e Monitor Sensor Connector Revised hardware software features package contents optional accessories and specifications in Ch 1 Moved Technical Support section from App A to Ch 1 and updated TAC addres...

Page 18: ...of Ch 5 including new section on liquid testing for ELx405 HT model Updated App A Troubleshooting and App B Error Codes added several Bio Stack error codes that appear on the ELx405 Added new Appendi...

Page 19: ...es and specs in Chapter 1 Introduction Revised artwork in Chapter 2 Installation Chapter 3 Operation and Chapter 4 Preventive Maintenance Revised instructions in Chapter 3 for the following Clarified...

Page 20: ...instrument may only be used for research and development or for other non clinical purposes Quality Control It is considered good laboratory practice to run laboratory samples according to instruction...

Page 21: ...apter to connect primary power to the ELx405 Use of a two prong adapter disconnects the utility ground creating a severe shock hazard Always connect the power cord directly to a three prong receptacle...

Page 22: ...tween 15 30 C Performance may be adversely affected if temperatures fluctuate above or below this range Storage temperature limits are broader see Chapter 4 Preventive Maintenance Caution Sodium Hypoc...

Page 23: ...pter 2 Installation for instructions Caution Waste Sensor Port The port on the back of all ELx405 Washers for the waste sensor is the same type as the 24 VDC power connector on some other Bio Tek prod...

Page 24: ...st Transient Burst EN 61000 4 5 1995 Surge Immunity EN 61000 4 6 1996 Conducted Disturbances EN 61000 4 11 1994 Voltage Dips Short Interruptions and Variations Directive 73 23 EEC Low Voltage Safety E...

Page 25: ...ute of Standards and Technology NIST Microplate Washers Dispense precision and average residual volume is traceable to NIST Specific data for a particular serial number is available on request from Bi...

Page 26: ...ll be required to correct the interference at his own expense Canadian Department of Communications Class A This digital apparatus does not exceed Class A limits for radio emissions from digital appar...

Page 27: ...h und Wechselstrom Corriente continua y corriente alterna Corrente continua e corrente alternata Earth ground terminal Borne de terre Erde Betriebserde Borne de tierra Terra di funzionamento Protectiv...

Page 28: ...t surface Attention surface chaude Warnen hei e Oberfl che Precauci n superficie caliente Attenzione superficie calda Consult instructions for use Consulter la notice d emploi Gebrauchsanweisung beach...

Page 29: ...cannot be assigned or transferred All claims under this limited warranty must be made in writing to Bio Tek Attention Service Department or to Bio Tek s authorized representative Purchaser must ship t...

Page 30: ...nt to fill out and mail the postage paid Warranty Registration card By sending in the registration card you will be assured of receiving prompt information on product enhancements Registration Online...

Page 31: ...ications Instructions on how to contact Bio Tek for Technical Assistance are included on page 12 Introducing the ELx405 2 ELx405 Washer Variations 3 Compatibility With the Bio Stack System 4 Hardware...

Page 32: ...upport Wash Prime Dispense and Aspirate programs In addition these programs can be linked together to run automatically An optional external valve switching module with four different supply bottles c...

Page 33: ...cond carrier is below the microplate the magnet will pull the beads into the bottom of the microwells and hold them there during aspiration of the supernatant and during soaking cycles The Magna can a...

Page 34: ...tematically transfer microplates one at a time to and from the washer and includes Removable stacks one input and one output that can hold up to 30 microplates each Optional restacking of plates to ma...

Page 35: ...Compatibility With the Bio Stack System 5 Figure 1 The ELx405 Microplate Washer With the Bio Stack ELx405 Operator s Guide...

Page 36: ...able through the on board software for low to high velocity dispensing 5 amp accessory outlet for automatic vacuum pump control Stepper motors for accurate and repeatable carrier and manifold position...

Page 37: ...washer program names resulting in the rapid recall of desired wash protocols and plate dimension set ups Programs may be copied edited or deleted Program parameters may be locked Requires custom prog...

Page 38: ...ld for HT models only that are sold with this accessory 7102064 7102068 Storage case for accessory 96 tube manifold 7102136 Models without Ultrasonic Advantage 7102033 Manifold shipping bracket Models...

Page 39: ...10 liter dispense bottle 7100559 10 liter waste bottle 7100557 Waste bottles 20 liter waste bottle 7100556 High Flow vacuum pump 1 3 HP 115 V used for applications with a 384 well microplate or where...

Page 40: ...o 85 non condensing Manifold Type Compatible with Single manifold with 96 sets of aspirate and dispense tubes arranged in an 8x12 array to process 96 well microplates ELx405 standard and Magna Two man...

Page 41: ...buffer equivalent is dispensed per well into a Costar 384 well flat bottomed plate The aspirate height adjustment is optimized for the plate prior to testing Dispense Precision Single Dual 96 Tube Man...

Page 42: ...name and serial number The software configuration information To see this information start at the washer Main Menu and follow the path below UTIL TESTS CHKSUM For troubleshooting assistance or instr...

Page 43: ...x with your questions or requests for help 24 hours a day to the following number Technical Assistance Center 802 655 3399 Written Communication If you prefer you may write a letter with your comments...

Page 44: ...ll based assays integrating a unique data reduction transformation or just looking for a recommendation on an appropriate fluorophore contact Bio Tek at the phone numbers or e mail address listed belo...

Page 45: ...te Washer installing its components and repackaging the instrument for shipment Unpacking and Inspecting the Instrument 16 Removing the Manifold Shipping Bracket 21 Setting Up the Washer 22 Verifying...

Page 46: ...securely packaged inside custom designed shipping materials This packaging should protect the instrument from damage during shipping Inspect the shipping box packaging instrument and accessories for s...

Page 47: ...Other Accessories HT2 HTV2 models with the 96 tube manifold The washer is shipped with the 192 tube manifold installed the 96 tube manifold is packaged in a special case and included in the accessori...

Page 48: ...caps and the plastic bag Figure 4 7 Save the end caps bag and shipping containers for repackaging 8 Note the manifold shipping bracket in Figure 5 the Select model is shown Follow the instructions on...

Page 49: ...a level surface 3 Remove any bubble wrap enclosing it 4 Lift out the two wire holders containing the four supply bottles the tubing and other accessories Figure 7 5 Remove the bubble wrap between the...

Page 50: ...e wrap Fluid ports 4 Figure 6 Removing the Valve Module From the Shipping Container Wire bottle holders 2 Supply bottles 4 Figure 7 Removing the Wire Holders Supply Bottles and Other Accessories From...

Page 51: ...stall the shipping bracket may void your warranty Do not discard the shipping bracket Refer to Figure 8 for the following the Select model is shown 1 Using the 9 64 3 57 mm hex wrench supplied unscrew...

Page 52: ...ched on the bottom side This serial number should match the washer s serial number If the numbers do not match call Bio Tek immediately Refer to Figure 9 for the ELx405 model Figure 10 for the Select...

Page 53: ...rasonic cleaner Manifold Carrier Carrier rail guides 2 Pin Carrier glide strips 2 do not remove Figure 9 Installing the Microplate Carrier for the ELx405 Model The ultrasonic cleaner is available in U...

Page 54: ...g trough or ultrasonic cleaner Manifolds 2 Pin Carrier Carrier rail guides 2 Slot Rail Figure 10 Installing the Microplate Carrier for the Select Select CW and all HT Models The ultrasonic cleaner is...

Page 55: ...ate carrier Slot Carrier rail guides 2 Manifold Pin Priming soaking trough Magnet plate carrier Carrier glide strips 2 do not remove Figure 11 Installing the Microplate and Magnet Plate Carriers for t...

Page 56: ...er all tubing cables and fittings for the waste and supply systems must be properly connected Figure 12 on the following page illustrates the rear panel of instrument and the locations of the ports an...

Page 57: ...mp accessory outlet Waste sensor port Fluid inlet fitting External valve module control port if installed Serial port Vacuum pump accessory fuse Power switch Power cable socket Vacuum port Figure 12 R...

Page 58: ...on Delay feature is available in the Setup utility that allows you to increase the delay thereby preventing the pump from drawing excess current and blowing the 5 amp fuse The delay should be set at 1...

Page 59: ...Vacuum pump Waste sensor cable from overflow bottle to waste sensor port Vacuum tube from waste bottle to vacuum port Vacuum tube from vacuum to waste bottles Waste overflow bottle Waste Waste bottle...

Page 60: ...uctions 1 To install the optional valve module connect the valve cable from the module to the valve control port on the back panel of the washer 2 Attach the tubing from one of the supply bottles to V...

Page 61: ...p the Washer 31 Supply bottles Supply tubing from supply bottle to fluid inlet port Supply Supply Figure 14 Washer With Supply Tubing Connections Without Optional External Valve Module ELx405 Operator...

Page 62: ...nected from valve module to fluid inlet fitting Supply bottles thin walled D C B A Supply Valve cable connected from valve module to valve control port Valve module Figure 15 Washer With Optional Exte...

Page 63: ...on top of the two posts shown in Figure 16 below and the two thumbscrew holes in the shield are lined up with the two holes in the front base of the washer 2 Insert the two thumbscrews and finger tigh...

Page 64: ...nect the system power cord directly to a three prong receptacle with a functional ground The ELx405 has a built in switching power supply that automatically adjusts for input voltage in the range of 1...

Page 65: ...ded Installation and Operational Qualification procedures to be performed after the instrument is installed and set up as described in this chapter and before the instrument is used in a laboratory en...

Page 66: ...io Stack and the PC controlling the Stacker An accessory package PN 7310010 that contains the alignment hardware for both instruments and a serial cable is included with the Bio Stack Please refer to...

Page 67: ...n PN 7103000 PN Outer shipping box 7102099 Inner shipping box 7102097 Left end cap 7102096 Right end cap 7102095 Valve module shipping materials PN Shipping box 3740008 Wire holder for buffer bottles...

Page 68: ...192 tube manifold is not installed install it now Store the 96 tube manifold in its special case 3 Run the PARK utility PARK prepares the manifold for installing the manifold shipping bracket UTIL SET...

Page 69: ...shut the outer box 6 Write the RMA number on the outside of the shipping box 7 Insure the instrument for full value before shipping to Bio Tek Repackaging the External Valve Module if equipped Refer t...

Page 70: ...40 Chapter 2 Installation Bio Tek Instruments Inc...

Page 71: ...its software ELx405 Front Panel 42 Keypad Description 43 Entering Program Names 44 System Startup 45 Main Menu 46 Programs 47 Operation With the Bio Stack 48 General Usage Guidelines 49 Wash 52 Prime...

Page 72: ...atures a 25 key keypad and a 2 line x 24 character LCD display as shown below Figure 17 ELx405 Front Panel With LCD and Keypad Alphanumeric keys Special function keys Menu functions second line of LCD...

Page 73: ...a washer program press the Main Menu key and then press YES to save the program To move to a previous menu press the Previous Screen key Press the W reverse arrow to move the cursor to the left in th...

Page 74: ...the cursor within the display Press the ENTER key to store the completed program name and continue If the program name already exists the washer software will require you to enter a unique name This p...

Page 75: ...If the Self Test fails the washer will beep and the display will show an error code Note the error code and then press the STOP key to stop the beeping Refer to Appendix A Troubleshooting and Appendix...

Page 76: ...tions BIO TEK ELx405 RUN DEFINE MAINT UTIL SELECT PROGRAM TYPE WASH PRIME DISP MORE SELECT PROGRAM TYPE ASPIR SOAK LINK MORE SELECT MAINT PROGRAM See options below SELECT UTILITY TESTS SETUP AUTCL AUT...

Page 77: ...g begins 73 DISPENSE Create a dispense only program to dispense a user specified volume into all wells in a microplate 79 ASPIRATE Create an aspirate only program to evacuate all wells in a microplate...

Page 78: ...Bio Stack Operator s Manual for setting up and configuring the washer to operate with the Bio Stack You can run Wash Dispense Aspirate and Link programs whether the washer is operated with or without...

Page 79: ...le installed and the waste detection sensor activated refer to Sensor System page 91 If fluid collects in the overflow bottle thoroughly rinse the level switch assembly and bottle Check the external t...

Page 80: ...d Bottles Plate carrier system Manifold Aspirate dispense tubes Mist shield Inlet filter Models with the Ultrasonic Advantage ultrasonic cleaner An AUTOCLEAN program should be run on a periodic basis...

Page 81: ...ming with air instead of fluid See Sensor System SENSOR p 91 Manifold Type HT models with the dual 96 tube manifold HT2 HTV2 and Select models upgraded to the HT The correct manifold 96 or 192 must be...

Page 82: ...IN CARRIER AND PRESS START KEY WASH PROGRAM RUNNING PRESS STOP KEY TO QUIT WASH PROGRAM COMPLETE NEW REPEAT Run Wash Program Options Option Description Models Action Comments Select Wash Program Selec...

Page 83: ...name for the Wash program All Use up to 16 alphanumeric characters See Entering Program Names on page 44 for instructions Select Reagent Bottle Select the supply source for this program All Select bot...

Page 84: ...e microwells after the buffer has been dispensed Soak Duration Specify the time to allow the wash buffer to remain in the wells before aspiration All The duration range is from 0 to 600 seconds A soak...

Page 85: ...s All The volume range is 1 to 999 ml Prime Flow Rate The rate at which the priming fluid is pumped into the dispense tubes All The flow rate options range from 1 to 9 1 Slowest and 9 Fastest Press Ma...

Page 86: ...for the Dispense Flow Rate see above The volume range is 50 to 3000 l well Bio Tek recommends that you set the vacuum on volume equal to your dispense volume for small volumes Refer also to applicati...

Page 87: ...ith a low flow rate are the recommended settings for gentle washing such as required by cell based assays To visually determine the best offset parameters for a microplate or assay use the Adjust Util...

Page 88: ...between the bottom of the aspirate tubes and the carrier surface on which the plate rests during the wash See Figures 18 to 20 All The Bottom Disp Height options range from 12 to 180 The washer displ...

Page 89: ...nges Parameter Units Default Range Models 50 3000 ELx405 Magna Select Select CW HT 96 tube Dispense Volume In a Dispense only program the default volume is 250 l well 300 25 3000 HT 192 tube 1 9 All D...

Page 90: ...Position Select Select CW HT only quarter steps 0 10 to 20 with 384 well plates Select Select CW HT 96 tube HT 192 tube Bottom Wash First No No Yes Bottom Dispense Volume l well 300 50 3000 Bottom Flo...

Page 91: ...elect Select CW HT 96 tube Bottom Horizontal Y Dispense Position Select Select CW HT only quarter steps 0 10 to 20 with 384 well plates Select Select CW HT 96 tube HT 192 tube Prime Before Start No No...

Page 92: ...W HT 96 tube The range is 55 to 55 with 96 well plates Select Select CW HT 96 tube HT 192 tube The range 25 to 25 with 384 well plates Note For higher throughput of the HT with 384 well plates use the...

Page 93: ...ate The rate at which the washer manifold travels down into the microwells while aspirating fluid For most assays non cell based a rate from 1 to 5 where 1 slowest and 5 fastest is sufficient With any...

Page 94: ...ise aspiration if the horizontal aspiration position is programmed correctly Select YES to designate a second aspiration Example of initial and then crosswise aspiration in a microwell Horizontal Aspi...

Page 95: ...pr Delay range is 0 to 5000 milliseconds Press Main Menu to save All Select YES to save the program Note The shaded options above are not available for Aspirate only programs Wash ASPIRATE Defaults an...

Page 96: ...seconds 0 0 5000 Crosswise Aspirate No No Yes All Crosswise On Final Final All All Crosswise Height 24 12 180 All 30 to 30 ELx405 Magna 55 to 55 with 96 well plates Select Select CW HT 96 tube Crosswi...

Page 97: ...Select CW HT quarter steps 0 10 to 20 with 384 well plates Select Select CW HT 96 tube HT 192 tube Final Aspirate Yes Yes No Final Aspirate Delay milliseconds 0 0 to 5000 All Notes The shaded options...

Page 98: ...pirate height Carrier mounting surface Dispense tubes Aspirate tubes Microplate Manifold Dispense height overflow position shown Figure 18 Dispense and Aspirate Heights for the ELx405 and Magna Models...

Page 99: ...d Aspirate height Bottom manifold Top manifold Microplate Carrier mounting surface Aspirate tubes Dispense tubes Bottom manifold Figure 19 Dispense and Aspirate Heights for the Select Select CW and HT...

Page 100: ...manifold Aspirate tubes Aspirate height Bottom manifold Top manifold Dispense height overflow position shown Carrier mounting surface Microplate Dispense tubes Figure 20 Dispense and Aspirate Heights...

Page 101: ...dels with the accessory 96 tube manifold installed Copy Wash Program To copy a Wash program follow the menu path shown below Some of these items are options and some are prompts The options are descri...

Page 102: ...n the table below DEFINE SELECT ACTION DELETE SELECT PROGRAM TYPE WASH SELECT WASH PROGRAM OK TO DELETE Delete Wash Program Options Option Description Models Action Comments Select Wash Program Select...

Page 103: ...options and some are prompts The options are described in the table below RUN SELECT PROGRAM TYPE PRIME SELECT PRIME PROGRAM CONNECT REAGENT BOTTLE AND PRESS START KEY PRIME PROGRAM RUNNING PRESS STO...

Page 104: ...cription Models Action Comments Select Reagent Bottle Select the supply source for this program All Select bottle A B C or D Only appears if the washer is equipped with the external valve module Name...

Page 105: ...ispensed to the trough The vacuum pump is turned off and the tubes are lowered into the trough to soak After the specified Soak Duration the vacuum pump is turned on and the trough is aspirated Soak D...

Page 106: ...l the lines are empty or when changing fluids Washers without the valve module 200 ml Prime Volume 150 ml Low Flow Prime Volume When all the lines are empty Select CW 300 ml Prime Volume 200 ml Low Fl...

Page 107: ...new Prime program All Use up to 16 alphanumeric characters See Entering Program Names on page 44 for instructions OK To Copy All Select YES to copy the program Delete Prime Program To delete a Prime...

Page 108: ...oes not prime the Select CW model s Low Flow tubing Parameter Units Default Models Prime Volume milliliters 200 All Low Flow Prime Volume milliliters 0 Select CW Prime Flow Rate 7 Soak After Prime No...

Page 109: ...h shown below Some of these items are options and some are prompts The options are described in the table below RUN SELECT PROGRAM TYPE DISP SELECT DISP PROGRAM PRIME THE WASHER NUMBER OF STRIPS PLACE...

Page 110: ...K TO SAVE PROGRAM The following options appear only on the specified models SELECT REAGENT BOTTLE Washers equipped with the external valve module excludes earlier models of the Select CW PLATE TYPE Se...

Page 111: ...ls with the accessory 96 tube manifold installed Copy Dispense Program To copy a Dispense program follow the menu path shown below Some of these items are options and some are prompts The options are...

Page 112: ...the table below DEFINE SELECT ACTION DELETE SELECT PROGRAM TYPE DISP SELECT DISP PROGRAM OK TO DELETE Delete Dispense Program Options Option Description Models Action Comments Select Dispense Program...

Page 113: ...follow the menu path shown below Some of these items are options and some are prompts The options are described in the table below RUN SELECT PROGRAM TYPE MORE ASPIR SELECT ASPIR PROGRAM PLACE PLATE I...

Page 114: ...nifold installed MAGNET ASPIR HEIGHT Magna HORIZ Y ASPR POS and CROSS HORIZ Y POS Select HT and Select CW Define Aspiration Program Options See Define Wash ASPIRATE ASPIR Program Options page 62 for a...

Page 115: ...or the new Aspiration program All Use up to 16 alphanumeric characters See Entering Program Names on page 44 for instructions OK to Copy All Select YES to copy the program Delete Aspiration Program To...

Page 116: ...ine a Soak program follow the menu path shown below Some of these items are options and some are prompts DEFINE SELECT ACTION CREATE SELECT PROGRAM TYPE MORE SOAK NAME SOAK DURATION SHAKE BEFORE SOAK...

Page 117: ...or the new Soak program All Use up to 16 alphanumeric characters See Entering Program Names on page 44 for instructions OK to Copy All Select YES to copy the program Delete Soak Program To delete a So...

Page 118: ...ied within the Link program however can be edited Run Link Program Important Before running a Link program ensure that you have reviewed the General Usage Guidelines on page 49 and that the microplate...

Page 119: ...er a unique name for the new Link program All Use up to 16 alphanumeric characters See Entering Program Names on page 44 for instructions Select Select CW Magna HT 96 tube Choose 96 or 384 Note For hi...

Page 120: ...he table below DEFINE SELECT ACTION DELETE SELECT PROGRAM TYPE MORE LINK SELECT LINK PROGRAM OK TO DELETE Delete Link Program Options Option Description Models Action Comments Select Link Program Sele...

Page 121: ...u path below The default baud rate for the washer is 9600 UTIL SETUP RS232 SELECT BAUD RATE 9600 1200 2400 9600 VIEW Sensor System SENSOR The SENSOR feature allows the user to activate or deactivate t...

Page 122: ...ion ASPIR aspiration DISP dispense or MAGNET magnetic plate Magna model only Only one tube position may be viewed at a time 5 At the AXIS selection screen choose an axis The top line of the display in...

Page 123: ...manifold shipping bracket To park the washer start at the Main Menu and select the following UTIL SETUP MORE PARK The manifold will immediately move to the Park or pre defined position Bio Stack Utili...

Page 124: ...Dissipation Delay feature enables you to change the value for vacuum dissipation delay at the completion of a program The delay range is 1 to 50 sec with a default value of 5 sec Increasing the delay...

Page 125: ...parameter is called Manifold Selection and it can be set to 96 or 192 For the models described above this parameter must be changed each time the manifold is changed For example the HT2 HTV2 is shippe...

Page 126: ...96 Chapter 3 Operation ELx405 Washer Menu Maps Refer to the Washer Menu Maps on pages 97 through 100 for a complete listing of options available through the Main Menu Bio Tek Instruments Inc...

Page 127: ...NTENSITY SOAK DURATION DISPENSE HEIGHT ASPIRATION RATE METHOD DISP ASPIR HORIZONTAL DISP POS ASPIRATE DELAY NUMBER OF CYCLES DISPENSE VOLUME ASPIRATE HEIGHT HORIZ Y DISP POS CROSSWISE ASPIR WASH FORMA...

Page 128: ...OGRAM NAME ENTER PROGRAM NAME ENTER PROGRAM NAME ENTER PROGRAM NAME OK TO COPY YES NO OK TO COPY YES NO OK TO COPY YES NO OK TO COPY YES NO OK TO COPY YES NO OK TO COPY YES NO RUN DEFINE MAINT UTIL CR...

Page 129: ...PROGRAM COMPLETE PRIME PROGRAM COMPLETE DISP PROGRAM COMPLETE ASPIR PROGRAM COMPLETE LINK PROGRAM COMPLETE NEW REPEAT NEW REPEAT NEW REPEAT NEW REPEAT NEW REPEAT If the NEW option is selected at the W...

Page 130: ...JUST PARK BIOSTACK DISP PLATE VACUUM MANIFOLD MANIFOLD SELECTION 1200 2400 9600 VIEW FIXED RS 232 SETTINGS VACUUM WASTE FLUID FLOW PLATE TYPE ADJUSTMENT POSITION AXIS POS MAN CARX CARY MANIFOLD DESCEN...

Page 131: ...t and decontaminate the instrument Overview 102 Recommended Maintenance Schedule 103 Required Materials 104 Warnings Precautions 105 Maintenance Programs 106 Prime Programs 107 Rinse Soak the Fluid Pa...

Page 132: ...can clog quickly and therefore they must be rinsed frequently and cleaned regularly Is the washer in use continuously or does it sit idle for several hours or days at a time If the washer will be sit...

Page 133: ...Clean Components Check empty waste bottle p 115 Clean bottles p 115 Clean plate carrier system p 115 Clean carrier transport arm p 116 Clean manifold and mist shield p 118 Clean aspirate and dispense...

Page 134: ...64 3 57 mm hex wrench supplied with the washer Lint free disposable towels Dish soap or other mild cleaner Soft bristled brush For replacing o rings and channel end seals O rings PN 49941 Channel end...

Page 135: ...ith Bio Tek part number 68098 Teflon valves Contact Bio Tek for upgrade information and prior to using other questionable chemicals Warning Wear protective gloves when handling contaminated instrument...

Page 136: ...select MAINT 2 Press the Options key to scroll through the available programs Press the ENTER key to select a program 3 If prompted for additional information such as RINSE VALVE for models with the e...

Page 137: ...Select CW model contains smaller diameter low flow tubing for gentle cell washing The Low Flow Prime Volumes specified in the charts below and on the following page are necessary to purge the Select...

Page 138: ...P_DECON1 Parameter Default Selectable Range Prime Volume 400 ml All models except Select CW 300 ml Select CW 1 999 ml Low Flow Prime Volume Select CW models only 200 ml 1 999 ml Flow Rate 7 1 9 Soak...

Page 139: ...solution does not contain surfactant consider rinsing at least once an hour The DAY_RINSE and AUTOPRIME programs described below and on the following page satisfy daily maintenance requirements DAY_RI...

Page 140: ...cate how often you want the AUTOPRIME Prime program to run 3 Press ENTER and then return to the Main Menu AutoPrime is now enabled and priming will occur each time the washer has been idle for interva...

Page 141: ...be left idle for two or more days run DAY_RINSE and OVERNIGHT_LOOP or run RINSE_AND_SOAK Important Keep the manifold in a wetted condition to ensure proper evacuation of fluid Bio Tek recommends chan...

Page 142: ...ing proteins such as bovine serum albumin BSA will compromise the washer s performance over time unless a strict maintenance regime is adhered to Do not use isopropyl alcohol to flush out BSA Bio Tek...

Page 143: ...nger than 45 minutes flush the proteins from the washer 1 Fill a supply bottle with deionized water Connect the bottle to the washer Valve A if the valve module is connected 2 Run the P_DAY_RINSE Prim...

Page 144: ...se components Important When cleaning components Do not immerse the instrument spray it with liquid or use a wet cloth on it Do not allow the cleaning solution to run into the interior of the instrume...

Page 145: ...rier System If liquid has overflowed onto the plate carrier transport rail or glide strips some buildup may occur and prevent the microplate from seating correctly on the carrier This can interfere wi...

Page 146: ...sary to disassemble and clean the spring loaded carrier transport arm that allows the Select Select CW and HT models to move forward and back along the y axis when washing 384 well plates If reagent h...

Page 147: ...arrier noted with an arrow in the photo below Figure 23 Removing the Screws and Washers From the Transport Arm 3 Remove the screws and washers and slide the arm free from the carrier Take note of the...

Page 148: ...erform a thorough cleaning of the dispense and aspirate tubes and channels When necessary follow the DECONTAMINATION procedure on page 130 to disinfect the manifold and tubing To clean the manifold mi...

Page 149: ...the manifold and end plates making sure that the two o rings are in place prior to reassembly Do not overtighten the manifold screws 11 Re attach the mist shield finger tighten the two thumbscrews use...

Page 150: ...rings 2 Manifold o rings 2 Channel end seals Manifold end plates 2 Carrier pin Carrier transport rail Slot for carrier pin Carrier glide strips 2 do not remove Carrier rail guides 2 Figure 25 Carrier...

Page 151: ...ing trough or ultrasonic cleaner Manifolds 2 Slot for carrier pin Figure 26 Carrier and Manifold Placement in the Select Select CW and HT Models The ultrasonic cleaner is available in Ultrasonic Advan...

Page 152: ...ws washers and springs 2 Channel end seals Manifold end plates 2 Priming trough Carrier for magnetic plate Manifold o rings 2 Microplate carrier Carrier pin Figure 27 Carrier and Manifold Placement in...

Page 153: ...vapor bubbles to be created The formation and subsequent collapse of these bubbles is the mechanism that cleans manifold tubes submerged in the bath The cleaner consists of a stainless steel reservoi...

Page 154: ...y bottle s An AUTOCLEAN program initially primes the system with 200 ml of fluid from the supply bottle specified for Loop 1 each cleaning loop fills the reservoir with approximately 93 ml of fluid Pr...

Page 155: ...e module fluid is dispensed and the next loop begins This process is repeated until all loops are complete Models without the valve module If more than one cleaning loop is defined the washer will pau...

Page 156: ...nel of the instrument To clean the filter 1 Unscrew the fluid inlet fitting from the back of the washer see Figure 28 2 Note the orientation of the filter in the fitting the cone shaped end of the fil...

Page 157: ...Clean Components 127 Vacuum port Fluid inlet filter Figure 28 Cleaning the Fluid Inlet Filter ELx405 Operator s Guide...

Page 158: ...ures are adequate for the Biohazard s they handle Warning Internal Voltage Turn off and unplug the instrument for all decontamination operations Do not immerse the instrument spray it with liquid or u...

Page 159: ...erum albumin 3 Moisten a cloth with the bleach solution or alcohol Do not soak the cloth Wipe the keypad do not soak Wipe again with a clean cloth moistened with deionized or distilled water Dry immed...

Page 160: ...bleach you are using this information is printed on the side of the bottle Commercial bleach is typically 10 NaClO if this is the case prepare a 1 20 dilution Household bleach is typically 5 NaClO if...

Page 161: ...INATION CONNECT DISINFECTANT AND PRESS START KEY RUNNING DECONTAMINATION DISINFECTING CONNECT RINSE BOTTLE AND PRESS START KEY Washer equipped with the external valve module MAINT SELECT MAINT PROGRAM...

Page 162: ...f the two posts Refer to Decon Procedure for External Surfaces of the Instrument on page 129 for decontamination of the mist shield Use extra care when removing the Select HT and Select CW dual manifo...

Page 163: ...bscrew holes in the shield are lined up with the two holes in the front base of the washer Refer to Figure 16 in the section Attaching the Mist Shield Chapter 2 Insert the two thumbscrews and finger t...

Page 164: ...nd Soak Durations specified in the P_DECON1 and P_DECON2 Prime programs These prime programs can be edited for optimum cleaning Refer to the charts in the Prime Programs section of this chapter for a...

Page 165: ...TANT AND PRESS START KEY Washers equipped with the external valve module MAINT SELECT MAINT PROGRAM LONG_SHUTDOWN DISINFECTANT VALVE A RINSE VALVE B AIR VALVE C START MAINTENANCE YES Storing the Washe...

Page 166: ...ved 2 Replace the used o rings with new ones by fitting them into the grooved slots 3 If you are not replacing the channel end seals at this time reinstall the manifold Do not overtighten the manifold...

Page 167: ...se any parts of this mechanism Lubricate the seals and the bored holes with alcohol to assist with reinsertion 3 Clean the seals if you are not replacing them with new ones 4 Lubricate both the seals...

Page 168: ...138 Chapter 4 Preventive Maintenance Bio Tek Instruments Inc...

Page 169: ...pter describes how to verify the performance of the ELx405 Microplate Washer Overview 140 Verification Schedule 141 Installation Qualification IQ 142 Operational Qualification OQ 142 Performance Quali...

Page 170: ...as the vacuum manifold and carrier positioning The Checksum Test verifies the basecode software against internal checksum values to ensure that no corruption has occurred If the Checksum Test is run...

Page 171: ...nse Precision with valve module Note Refer to page 147 to determine which tests to perform Important The risk factors associated with your assays may require that the Operational and Performance Quali...

Page 172: ...ially and over time The recommended OQ procedure consists of performing the System Self Test and the Checksum Evacuation Efficiency and Dispense Precision tests Your facility s operating policies may...

Page 173: ...recision tests Your facility s operating policies may also require that you routinely perform an actual assay to confirm that the washer will consistently give adequate results for the assays to be ru...

Page 174: ...mmand is executed If the magnet holder loses steps when making these moves the position verify will generate an error code failing the test To run the System Self Test At the Main Menu select UTIL TES...

Page 175: ...ecode software part number version number and checksum 7100xxx Version x xx CODE CHECKSUM xxxx After a few seconds a second screen will display the assay software part number and version number 7100xx...

Page 176: ...o evacuated from the wells and the plate is weighed Buffer is then pipetted to all wells to bring the volume of fluid to a consistent measurement level The optical density of each well is measured at...

Page 177: ...ncy Test Dispense Precision Test without Valve Module 150 153 405 Magna Single 96 Yes Evacuation Efficiency Test Dispense Precision Test with Valve Module 150 154 No Evacuation Efficiency Test Dispens...

Page 178: ...capable of dual wavelength reading at 630 450 nm Liquid Test Worksheets at the end of this chapter for recording data and results Deionized water Test solutions Prepare these solutions using the recip...

Page 179: ...1420 ml of deionized water with 20 ml of Solution 2 You may use your own buffer solution in place of Solution 1 If any tests fail using your own buffer however retry the tests using the suggested solu...

Page 180: ...small amount of residual fluid 5 When the program is finished remove the plate and weigh it immediately because evaporation will affect the results This is the Total Residual Weight in grams 6 Visual...

Page 181: ...ndard Evacuation Efficiency Test fails twice This test will confirm which aspirate tube s may be clogged or if the plate s alignment or position is the problem 1 Repeat steps 2 through 7 of the previo...

Page 182: ...AUTOCLEAN Ultrasonic Advantage models only and or remove the manifold and thoroughly clean the tubes with the stylus all models See Chapter 4 Preventive Maintenance for complete instructions When fin...

Page 183: ...e program is finished carefully remove the plate If you are using one of Bio Tek s keypad based readers such as the ELx800 or ELx808 ensure that the reader is not running in Rapid mode To check the se...

Page 184: ...on the washer and run the Dispense program accuracy_qc_test This program dispenses 300 l of solution to each well of the plate It does not evacuate the solution 5 When the program is finished carefull...

Page 185: ...er Place the bottles on a surface level with the washer i e the adjacent lab bench area Repeat the following steps for each valve 3 Run the Maintenance program DAY_RINSE two or three times to prime th...

Page 186: ...to fail contact Bio Tek 8 Record the results in the Dispense Precision Test Worksheet on page 166 For the ELx405 standard model only the accuracy_qc_test program is locked for editing it can only be...

Page 187: ...iquid in them than others 7 Using the Evacuation Efficiency Test Worksheet on page 167 perform initial data reduction a Divide the Total Residual Weight by 384 to find the Mean Residual Weight b The M...

Page 188: ...olution 3 Shake the plate to get uniform distribution of the remaining dye in each well Note If you are using one of Bio Tek s keypad based readers such as the ELx800NB ensure that the reader is not r...

Page 189: ...tenance for complete instructions When finished retry the test If the problem appears to be related to a particular region edge or corner of the plate review the alignment and flatness of the plate on...

Page 190: ...ion 5 When the program is finished carefully remove the plate Note If you are using one of Bio Tek s keypad based readers such as the ELx800NB ensure that the reader is not running in Rapid Mode To ch...

Page 191: ...microplate on the washer and run the Dispense program 192accuracy_qc This program dispenses 80 l of solution to each well of the plate It does not evacuate the solution 5 When the program is finished...

Page 192: ...needed throughout this procedure 2 Fill each of the supply bottles connected to Valves A B C and D with three liters 3000 ml of deionized water Repeat the following steps for each valve 3 Run the Main...

Page 193: ...aning the filter The valve should be cleaned using an appropriate enzyme alcohol or a diluted bleach solution depending on the contaminant See Removing Protein Residuals and Fungi Growth and Decontami...

Page 194: ...esidual Weight g Verification that wells are consistent in appearance Pass Fail Mean Residual Weight Total Residual Weight 96 g Mean Residual Weight 0 005 g Pass Fail Supplemental Test check here if n...

Page 195: ...ule Washer Model ELx405 Select CW Magna HT2 with Dual 96 manifold Select Serial Number Calculations Standard Deviation calculate using spreadsheet program Mean OD sum of all wells number of wells Coef...

Page 196: ...Select Serial Number Calculations for Valve A Standard Deviation Mean OD Sum of all wells Number of wells CV Standard Deviation Mean OD x 100 CV 4 0 Pass Fail Calculations for Valves A D Annual OQ on...

Page 197: ...istent in appearance Pass Fail Mean Residual Weight Total Residual Weight 384 g Mean Residual Weight 0 002 g Pass Fail Supplemental Test check here if not performed Mean OD for the plate sum of all we...

Page 198: ...ithout the External Valve Module Washer Model HT HT2 with Dual 192 manifold Serial Number Calculations Standard Deviation calculate using spreadsheet program Mean OD sum of all wells number of wells C...

Page 199: ...Valve A Standard Deviation Mean OD Sum of all wells Number of wells CV Standard Deviation Mean OD x 100 CV 4 0 Pass Fail Calculations for Valves A D Annual OQ only check here if not performed Total Di...

Page 200: ...170 Chapter 5 Performance Verification Qualification Tests Bio Tek Instruments Inc...

Page 201: ...Appendix A Troubleshooting This appendix lists problems that you may experience with the washer and suggests possible solutions for these problems Overview 172 Troubleshooting Charts 173...

Page 202: ...p accessory cable waste supply tubing and fittings Incomplete or incorrect installation of manifold carrier High Flow vacuum pump external valve module incorrect attachment of mist shield or placement...

Page 203: ...unter problems during plate transfers between the Stacker and the washer or during communication between the two instruments refer to the Troubleshooting and Error Codes section of the Bio Stack Opera...

Page 204: ...d not into a wall outlet See Ch 2 Installation Manifold or carrier is being obstructed Remove obstruction Motor sensor or electrical problem Turn washer off wait at least 15 seconds then turn back on...

Page 205: ...n the washer manifold Remove and clean the manifold see Cleaning the Manifold in Ch 4 Preventative Maintenance Microplate aspiration height adjustment too high or too low Change the Aspiration Height...

Page 206: ...Enable crosswise aspiration 2 Increase aspiration time to 800 ms 3 Slow aspiration speed to 1 or 2 Aspiration tubes not properly positioned horizontally in wells If none of the tubes are bent try adj...

Page 207: ...the Plate Carrier System in Ch 4 Try a different microplate or strip holder If the problem is unresolved the carrier may have to be realigned Contact Bio Tek TAC Washer program requires optimization...

Page 208: ...do not use the AutoPrime program for the initial prime All models except Select CW 200 ml Prime Volume when all the lines are empty or when changing fluids Select CW models 200 ml Prime Volume and 150...

Page 209: ...w flow rate 10 or 11 used with 384 well plates Select a higher flow rate see Define Wash DISPENSE Program Options in Ch 3 Aspiration tubes hit bottom of trough during Prime or Maintenance Manifold may...

Page 210: ...me Volumes do not use the AutoPrime program for the initial prime All models except Select CW Without the valve module 200 ml Prime Volume when all the lines are empty or when changing fluids With the...

Page 211: ...cuum filter check the filter for clogging and replace if necessary Check seal of waste bottle covers Check for air leaks in the waste tubing and bottles Use a slower Aspiration Rate in the program see...

Page 212: ...Options in Ch 3 Aspiration tubes not entering well correctly Aspirate tubes bent Contact Bio Tek TAC Washer Manifold Movement Problem Possible Cause Possible Solution Manifold movement is blocked Chec...

Page 213: ...e Wash DISPENSE or ASPIRATION Program Options in Ch 3 Scratches on microplate bottom Microplate not properly seated or strips not level Reseat microplate in carrier or strips in holder Make sure the c...

Page 214: ...184 Appendix A Troubleshooting Bio Tek Instruments Inc...

Page 215: ...may appear on the ELx405 Microplate Washer If an error is displayed refer to Appendix A Troubleshooting Contact Bio Tek s Technical Assistance Center if further instructions are necessary Error Codes...

Page 216: ...rror is displayed refer to Appendix A Troubleshooting Contact Bio Tek s Technical Assistance Center if further instructions are necessary Bio Stack Errors Error codes displayed on the washer during op...

Page 217: ...m errors n program Code Cause 0100 ABORT_ERR Washer function was aborted due to operator initiated abort or following some other error 0200 NO_SENSOR_ERR Manifold motor couldn t find the optical senso...

Page 218: ...Causes Carrier x axis motor is unable to move due to contamination of the carrier transport rail Carrier x axis motor failed belt is slipping or drive or idler pulley is defective Optical sensor is d...

Page 219: ...ual manifold models only Carrier y axis motor couldn t find edge of autocal jig Probable Causes Autocal jig contacts are contaminated Autocal jig is not properly installed or a connection is not made...

Page 220: ...ify occurs when verifying the current motor position during self test prior to running a program or following a program Probable Causes Carrier x axis motor is unable to move due to contamination of t...

Page 221: ...emory e g when saving a program via the washer keypad or when downloading programs from a controlling PC and there are no empty locations Probable Causes Processor failure 0800 PROGRAM_DEL_ERR Invalid...

Page 222: ...gram Probable Causes Incorrect rate volume entered for manifold selected 0F00 DISPENSE_VOLUME_ERR Dispense volume invalid for manifold type occurs when verifying the validity of a program e g during r...

Page 223: ...s requested to be moved before it has been homed Probable Causes Errors 0201 or 0401 initially occurred and were not resolved see the Probable Causes for 0201 1302 MOTOR_NOT_HOMED_ERR Magna models onl...

Page 224: ...ontal aspirate position requested is out of range See Wash ASPIRATE Defaults and Ranges in Chapter 3 Operation for a list of acceptable ranges 1B00 DISPENSE_POS_ERR Horizontal dispense position is out...

Page 225: ...pump fuse has blown or external pump is defective and unable to reach minimum vacuum requirements of 1 6 HG Vacuum source has not reached 1 6 HG Waste bottle cap or caps are loose Vacuum switch has f...

Page 226: ...the dispense operation Probable Causes Fluid or sensor housing is opaque not allowing the infrared detector to toggle Infrared detector or fluid sensor failed Main PCB failed to detect the fluid sens...

Page 227: ...d not able to float Infrared detector or fluid sensor has failed Main PCB failed to detect the fluid sensor Air bubble in the detection path No fluid in unit unit has not been primed Stuck or defectiv...

Page 228: ...or turning the vacuum on and off the axis or sensor errors would be displayed first before a 3100 error Probable Causes One of the axes failed Vacuum switch did not close before vacuum delay expired...

Page 229: ...of range loop valve index 3A00 AUTCLN_VOLUME_INVALID_ERR Invalid fill volume value entered This occurs when trying to start the AUTOCLEAN feature and the value stored in the flash memory for the fill...

Page 230: ...to create a new software task to run simultaneously with other tasks such as when first trying to run a wash program or when trying to start an AUTOCLEAN program in an Ultrasonic Advantage model A300...

Page 231: ...are thinks the motor is still running even if it isn t A304 NOT_AVAIL_ERR Software timer not available A305 NOT_AVAIL_ERR Display not available A306 NOT_AVAIL_ERR Quick flash memory not available A307...

Page 232: ...low configuration downloads saving of autocal data or program files etc A800 RAM_ERR RAM error occurs during failure of the RAM test on the test station EPROM test station error only A900 HEAP_CORRUPT...

Page 233: ...Bio Stack B 8304 NAK_RECEIVED_ERR The ELx405 is unable to communicate with the Bio Stack because the Bio Stack didn t properly acknowledge a message sent to it This occurs when a NAK is received from...

Page 234: ...in a bad state and needs to be turned off and then turned back on The cable connection is lost not allowing communications to transfer properly B 8306 TIMEOUT_ERR The ELx405 failed to communicate wit...

Page 235: ...the name and material s of each washer component that will come into contact with chemicals during operation or maintenance If there are questions about compatibility of chemicals with the washer con...

Page 236: ...id pump 304 SS Dispense and aspirate tubes feeder tubes to manifold spring in bottle fittings 316 SS Flow sensor Polystyrene Outlet fitting fittings in bottles inline fittings ball Polypropylene Inlet...

Page 237: ...Appendix D Default Programs This appendix lists all default wash prime dispense aspirate and maintenance programs available on the washer Default Programs 208...

Page 238: ...AND_SOAK X X X X X P_DECON1 X X X X X P_DECON2 X X X X X Dispense accuracy_qc_test X X X X X 192accuracy_qc X Aspirate residual_qc_test X X X X X 192resid_qc_test X Maintenance DAY_RINSE X X X X X OVE...

Page 239: ...dix E Changing the Manifolds This appendix contains instructions for changing manifolds in ELx405 HT models with the accessory 96 tube manifold Changing Manifolds in HT Models With the 96 Tube Manifol...

Page 240: ...em dry From the main menu select UTIL SETUP SENSOR and set VACUUM DETECTION FLUID DETECTION AND FLOW DETECTION to NO Connect an empty supply bottle and run DAY_RINSE Important Set the vacuum fluid and...

Page 241: ...ield with the washer so it rests on top of the two posts and the two thumbscrew holes in the shield are lined up with the two holes in the base of the washer Insert the two thumbscrews and finger tigh...

Page 242: ...re lower manifold for dual 96 or 192 tube manifold in this compartment Store upper manifold for dual 96 or 192 tube manifold in this compartment Figure 30 Storing the 96 or 192 Tube Dual Manifold in t...

Page 243: ...6 Assays Cellular 2 ELISA 2 AUTOCLEAN Creating and running programs 124 125 Error codes 198 199 Warning xix AUTOPRIME 110 B Biohazards xix Bio Stack Compatibility with washer 2 4 Installation with was...

Page 244: ...ult programs 208 Dispense flow rate 56 59 Dispense height 56 59 Dispense Precision Test Defined 146 For 192 tube 160 For 96 tube 153 Valve module 154 161 Dispense program 79 Copy dispense program 81 D...

Page 245: ...Changing the manifolds 209 HT2 and HTV2 models 3 Humidity warning xix 22 I Installation Qualification IQ 142 Installation qualification package 35 Intended use Clinical xviii Research and development...

Page 246: ...8 Performance Qualification PQ 143 Performance specifications 11 Performance verification After installation 35 Periodic maintenance tasks 114 Plate carrier Cleaning 115 Plate type 53 Plate type prom...

Page 247: ...tion xxi Removing 112 R Registration Card xxviii Online xxviii Regulations 38 Remove magnet during shake 55 Repackaging and shipping Washer 37 Replace components 136 Replace channel end seals 136 Repl...

Page 248: ...bols using in program name 44 System Self Test 144 T TAC 12 Technical assistance center 12 Tests Checksum Test 145 Dispense Precision 192 tube 160 Dispense Precision 96 tube 153 Evacuation Efficiency...

Page 249: ...tage xix W Warranty xxvii Conditions for voiding 16 21 Wash format 54 55 Wash program 52 Copy wash program 71 Define wash aspirate program options 62 Define wash dispense program options 56 Define was...

Page 250: ...220 Index Bio Tek Instruments Inc...

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