Thermo Finnigan LCQ Deca XP Plus, Руководство по оборудованию

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User Maintenance
API Source Maintenance ______________________________________________________
4-8
____________________ LCQ Deca XP Plus Hardware Manual ________________
6. Set up and start a flow of 50:50 methanol/water solution from the LC to
the API source, as follows:
a. In the Tune Plus window, choose Setup | Direct Inlet Control (or
click on the LC pump button). The Inlet Direct Control dialog box
appears.
b. Select the LC tab.
c. In the Inlet Direct Control dialog box, set the Flow Rate to a value
that is typical for your experiments.
d. In the Inlet Direct Control dialog box, set the % spin boxes to 50%
methanol and water.
e. Click on Start to start the LC pump.
7. Let the solution flow through the sample transfer line, sample tube, and
API probe for 15 min. After 15 min, turn off the flow of liquid from the
LC to the API source, as follows. Leave the API source (including the
APCI vaporizer, sheath gas, and auxiliary gas) on for an additional 5 min.
a. In the Tune Plus window, click on the LC pump button. The Inlet
Direct Control dialog box appears.
b. Select the LC tab.
c. Click on Stop to stop the LC pump.
8. After 5 min, turn off the API source by placing the MS detector in
Standby as follows: From the Tune Plus window, choose Control |
Standby
(or click on the On/Standby button) to put the MS detector in
Standby .
Go on to the next topic: Flushing the Spray Shield, Ion Sweep Cone, Spray
Cone, and Ion Transfer Capillary.
Flushing the Spray Shield, Ion Sweep Cone,
Spray Cone, and Ion Transfer Capillary
You need to clean the spray shield, ion sweep cone, spray cone, and ion
transfer capillary on a regular basis to prevent excessive contamination and to
maintain optimum performance of your API source. A good practice is to
flush the spray shield, ion sweep cone, spray cone, and ion transfer capillary
at the end of each operating day after you flush the sample transfer line,
sample tube, and API probe with a 50:50 methanol/water solution from the
LC. (Refer to the topic Flushing the Sample Transfer Line, Sample Tube,
and API Probe on page 4-6.) If you are operating the system with
nonvolatile buffers in your solvent system or with high concentrations of
sample, you might need to clean the spray shield, ion sweep cone, spray cone,
and ion transfer capillary more often.