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24.5. Pipettes Calibration Start-Up
In order to carry out pipettes calibration process you need a special adapter, the adapter
does not come standard with the balance. The adapter facilitates piston pipettes calibration
and/or verification, it is intended for 3Y and 4Y balances.
The adapter has been designed to minimize evaporation occurring in the course of liquids
weighing. It comprises a mini weighing chamber installed inside the weighing chamber of a
balance, an evaporation ring and a dedicated weighing pan enabling to locate a measuring
vessel in the very centre.
Application of the adapter prevents risk of liquid evaporation during the calibration process.
Compact dimensions of the adapter along with use of the evaporation ring enable
maintaining high humidity rate inside the weighing chamber.
Carried out research has proved that the adapter eliminates or considerably reduces liquid
evaporation process. Eliminated/reduced liquid evaporation is a key factor while calibrating
piston pipettes by means of a gravimetric method.
Selecting an appropriate adapter and balance is conditioned by calibrated pipettes type.
Prior to pipettes calibration start install the adapter inside of the weighing chamber, next
enter data regarding pipettes that are to be calibrated, include complete pipette
characteristics and values of errors (see: Pipettes Database).
Now set pipettes calibration parameters:
•
Measurements quantity
•
Request serial number (YES/NO).
•
Operation with adherence to ISO 8655 (YES/NO)
•
Upload ambient conditions parameters from THB module (YES/NO).
CAUTION:
For parameters and settings overview refer to section 24.1 of this user manual.
Upon setting the above parameters, you can start pipettes calibration.
Pipettes calibration procedure:
1. Press <
Select pipette> button.
2. List of pipettes is displayed, select pipette to be calibrated.
3. Pipettes calibration home screen is displayed, selected pipette name is visible in INFO
field.
4. Press <
START> button.
5. With <YES> value set for <Request serial number> parameter a window for entering
the number opens.
6. Window for entering ambient conditions values is displayed, read the temperature,
humidity and pressure from external sensors and enter them, next press <
Accept> button.
With <YES> value set for <Upload ambient conditions parameters from THB module>
parameter, the temperature, humidity and pressure values are acquired automatically
from the THB module, water temperature must be entered manually, enter it and
press <
Accept> button to confirm.
7. Pipettes calibration home screen is displayed, tested volume and process status <In
progress> are displayed in INFO field. The task bar displays prompts guiding you
through the process - < C1/V1/N1 Sample weighing >.
Where: C1 – channel number; V1 – volume number for the channel; N1 –
measurement number for tested volume.
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