Fluorolog-3 v. 2.2 (10 Sep 2002)
Optimizing Data Acquisition
4-9
Enter
S/R
(Signal/Reference).
Using
S/R
produces a
spectrum corrected for
variations in lamp intensity
with respect to time.
Click
OK
to close the
Signals
dialog box.
6
Enter the remaining
parameters and, if
desired, the data file
name and the
experiment file
name, and any
comments desired that will distinguish this
experiment from others.
7
Click Run to execute the experiment.
The resulting spectrum displays intensity versus wavelength, and shows the
maximum excitation wavelength.
Optimized excitation and emission spectra of a 1 × 10
–8
-
M
anthracene solution are shown
below. Because the acquisition modes were different for the excitation and emission scans,
the data intensity had to be normalized. After normalization, notice that the excitation and
emission scans are virtually mirror images of one another.
Normalized excitation and emission spectra of a 1 × 10
–8
-
M
anthracene solution.
300 350 400 450 500
Wavelength (nm)
Intensi
ty (1
0
6
counts s
–1
)
4
3
2
1
0
Excitation
Emission
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