Fluorolog-3 v. 2.2 (10 Sep 2002)
Optimizing Data Acquisition
4-8
Try 250 nm.
Use the emission maximum from the previous pro-
cedure minus 15 nm. That is, if the emission maxi-
mum was 450 nm, use 435 nm.
Use the emission maximum
from the previous procedure.
Excitation scan
The next step is to use the recently discovered emission maximum to determine the optimal
excitation wavelength for the sample. The procedure is very similar to that outlined above.
1
On the
Run Experiment
toolbar, choose the
Experiment button:
As before, this opens the
Emission Acquisition
dialog box.
2
Click Exp Type.
This opens the
Select Experiment Type
dialog box.
3
Choose Excitation Acquisition.
Click
OK
to close the
Select Experiment Type
dialog box..
4
Specify the data acquisition parameters:
5
Click on the Signals... button.
This opens the
Signals
dialog box:
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
