Fluorolog-3 v. 2.2 (12 Jul 2002)
Technical Specifications
15-2
Spectrofluorometer system
The Fluorolog
®
-3 spectrofluorometer consists of modules and components controlled
by the specialized software. Although the system can be configured in various ways for
a variety of applications, the basic (standard) Fluorolog
®
-3 spectrofluorometer system
consists of the following components:
Excitation Source
450-W xenon short arc, mounted vertically in an air-cooled
housing. Light collection and focusing by an off-axis mirror for
maximum efficiency at all wavelengths.
Optional pulsed lamp or laser port interface available.
Spectrometers
Single-grating excitation and emission spectrometers (standard).
Spectrometers are Czerny-Turner design with kinematic classi-
cally-ruled gratings and all-reflective optics.
The following specifications are based on 1200-grooves/mm
gratings:
Resolution
0.2
nm
Accuracy
±0.5 nm
Speed
150 nm/s
Range
0–1300 nm
Gratings*
Excitation
330-nm blaze (200–700 nm range)
Emission
500-nm blaze (300–1000 nm range)
*Other gratings available for ranges above 1 µm.
Optional double-grating units available for highest stray-light re-
jection and sensitivity.
Sample Module
T-format sample module with excitation reference detector. The
T-box design allows a second emission-detection channel to be
incorporated. The sample module also has a removable gap-bed
assembly for sampling accessory replacement.
Optional front-face collection assembly available.
Detectors
Calibrated photodiode for excitation correction from 240–1000
nm. Emission detector is an R928P for high sensitivity in pho-
ton-counting mode (240–850 nm).
Other PMTs to 1100 nm, with thermoelectrically cooled option.
Solid-state detectors for higher wavelength emissions. CCD mul-
tichannel detector for instant emission spectra and sample spatial
information.
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