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V1.0

20170825

5. Use

5.8 Staining

Before staining program is started, the following steps must be prepared:

　 Optimize all menu (configuration interface) parameters.
　 Reagents must be configured for stations, and are injected according to

this configuration.

　Ensure that the program required by the staining can be started at any

time.

5.8.1 Loading slide stands

•

Slide stands can be loaded through the loading
station.

•

Open the drawer, and insert slide stands at the
loading station.

•

Close the drawer.

Pay attention to the operation sequence at the initial phase of oven

station.

The filing of reagent is forbidden during heating, or the heating operation

is forbidden after reagent filling at the oven station. The highest

temperature of oven can be 70

℃

. Reagent may catch fire, damaging the

apparatus and samples.

In order to correctly recognize slide stands, the

drawer must be opened for loading, and then

closed again. Do not open the transparent gate to

insert slide stands.

Fig.55

Содержание DP260

Страница 1: ...Dakewe Shenzhen medical equipment Co Ltd V1 0 Slide Stainer User s Manual Applicable models DP260 DP261 DP260s ...

Страница 2: ...ce The manual is an indispensable guideline for the use of the device This user s manual is a document matched with the machine and shall be placed at a place near the device so that it is convenient for relevant personnel or maintainers to view Prior to use please carefully read this manual and operate accordingly We believe it is of great help to your effective use of this device In case of any ...

Страница 3: ...eedback of the wrong description charts or technical illustrations contained in this manual so that we can update them It should be specially noted that we do not assume any responsibility on any of the direct or indirect economic loss or damages arising from the non conformance with the statement or other information of this manual This document is protected by copyright law and all copyrights of...

Страница 4: ... Technical Parameters 12 3 4 Station Modules 13 4 Installation 14 4 1 Site Requirements 14 4 2 Installation of the Apparatus 14 4 3 Connection of Water Inlet Pipe 15 4 4 Connection of Wastewater Discharge Pipeline 16 4 5 Apparatus Levelling 17 4 6 Electrical Connection 18 4 7 Transport 19 5 Use 20 5 1 Starting the Apparatus 20 5 2 Human Machine Interaction Interface Overview 21 5 3 User Log in 22 ...

Страница 5: ...6 5 8 Staining 57 5 8 1 Loading slide stands 57 5 8 2 Starting staining program 58 5 8 3 Viewing staining progress 60 5 8 4 Paused and Resume staining program 61 5 8 5 Pause staining program 62 5 8 6 Button indications of staining process stations 63 5 8 7 Completing staining procedure 63 6 Cleaning and Maintenance 64 6 1 Apparatus Cleaning 64 6 2 Regular Maintenance 65 7 Troubleshooting 66 7 1 Ov...

Страница 6: ...al Any other usage will be considered improper 1 3 Personnel Qualifications Only the trained laboratory personnel can operate the apparatus Operations on this apparatus can be performed only in accordance with the instructions of this manual This sign indicates the apparatus which becomes burning hot on its surface during operation 1 The number in the bracket refers to the serial number of the ite...

Страница 7: ... containing the apparatus serial number is attached on the back side of the apparatus Please conform to the instructions and warnings on safety specified in this chapter Even if you have been familiar with the operation and use of the product please read these instructions ...

Страница 8: ...econd responsible persons including operation maintenance or repair personnel In order to guarantee smooth operation of the apparatus conformity to the following instructions and warnings must be guaranteed Handling of reagents The disassembly or remodeling of the protective devices on the apparatus and its accessories is forbidden and only authorized maintainers can repair the apparatus and handl...

Страница 9: ...wer board without protective earth conductor The apparatus must be installed in a well ventilated area and shall be kept away from any fire source The chemicals used in slide stainer are inflammable and toxic The apparatus shall not be used in a room subject to explosion hazard If there is great temperature difference between the warehouse and apparatus installation place as well as high air humid...

Страница 10: ... pathway system power supply module and main body cover see Fig 1 and Fig 2 for details Fig 1 Dyeing cabinet Transparent Mechanical Protective shell Loading and unloading drawer 2 USB port Internet access Display screen Exhaust air filter screen Exhaust outlet Input of power jumper Input of main power 4 Foot pad Inflow water pressure regulating valve Water inlet pipe interface Water outlet pipe in...

Страница 11: ...and changes the oven station into reagent station Therefore the serial number of the station is R9 R13 The materials of both the reagent tank and other stations are PP DP260s disable the heating function of R9 R12 station and the material of reagent tank is PP Fig 3 Markers of staining cabin stations Reagent station Water washing station Reagent station Oven station Reagent station Loading station...

Страница 12: ...station 3 3 Technical Parameters Conventional Rated voltage 220V Rated frequency 50Hz Maximum power consumption 800VA GB 4793 1 classification Electric shock protection Class I Overvoltage category Class II Pollution grade Class II Non continuously running equipment Operating temperature range 15 to 40 Relative humidity 10 80 non condensing Size and weight Host L W Hmm 1066 x 665 x 580 Net weight ...

Страница 13: ...he staining stops after reagent filling please use the cover of the reagent tank 2 to reduce the evaporative capacity of reagent Water washing station The water washing station consists of four water washing tanks each of which can hold a slide stand Water enters the water washing station through the connector 3 of the bed plate and flows out from the overflow port 4 on the margin of the upper lef...

Страница 14: ...is almost no vibration on the floor Maintain environmental temperature stable between 15 and 40 The maximum relative humidity is 80 without condensation Do not place any vibration source that may induce vibration near the apparatus 4 2 Installation of the Apparatus Elevate the apparatus and hold the transport position of the apparatus Place the apparatus at the proper position of a workbench Check...

Страница 15: ...ly system Place the sealing pad ring 3 into the connector of the input end of water inlet pipe 8 connect it to the water inlet connector 9 of slide stainer and screw it tightly to ensure that the pipeline is sealed The regulator 10 can adjust the size of the water flow Before adjusting the water pressure first pull the blue control valve knob out and rotate it The direction rotates to increase the...

Страница 16: ...using the screwdriver until the pipe clamp 10 tightly fixes the drain pipe 12 on the connectors 11 Connect the other end of the drain pipe to the wastewater discharge system at the site connection method is subject to the design of the wastewater discharge system In case of the need of removal turn the screw 13 leftward using a screwdriver until the pipe clamp 10 releases the drain pipe 12 Then pu...

Страница 17: ...e adjustment use a monkey wrench 16 to release the stand bar and fasten the hexagon nut 14 see Fig 9 Place the cassette level gauge at four positions of the slide stainer A B C and D see Fig 10 Screw the apparatus stand bars inwards outwards according to actual needs to adjust the screw nut 15 and gradually adjust the apparatus to the horizontal level Start the adjustment from the apparatus stand ...

Страница 18: ...emote technical services upgrade and maintenance 22 23 24 Make sure that the four apparatus stand bars are stably placed on the workbench after apparatus adjustment is completed The apparatus must be connected to grounded main electrical outlet Fig 11 17 18 19 20 21 Fig 12 In case of the need for connecting UPS power use the UPS self contained power cable to connect the input and jumper input 18 p...

Страница 19: ...move all the slide stands Clear all reagent tanks Disconnect the apparatus power supply Remove water inlet pipe and drain pipe If it is necessary to transport the apparatus from a long distance place the apparatus into the original shipping box so that the apparatus is fixed and safe transportation is ensured The apparatus can be transported after being shut down ...

Страница 20: ...apter 5 2 Shutdown The system provides two shutdown methods soft shutdown and hard shutdown Soft shutdown When the apparatus has no running program press the shutdown button on the lower right side to close the human machine interaction system When the apparatus has a running program the machine cannot be shut down When the shutdown button is pressed the message program is running and the system c...

Страница 21: ...operations please refer to Section 5 3 User Log In and Section 5 5 5 User Soft shutdown only shuts down the upper computer and the lower computer is at the on status The machine can be completely shut down only by turning off the main switch on the left side If soft shutdown is not executed and hard shutdown is directly performed the upper computer and lower computer of the apparatus can be shut d...

Страница 22: ...on the right side of the User Name column and the drop down box of user list is displayed Click any of the users to choose a user Click the Code input column and the soft keyboard is displayed Enter the set password successively and click Confirmation to log in to the system Fig 15 or click Close and then click Log in Fig 15 ...

Страница 23: ...e level of authority of the selected user Overview of interface modules Fig 16 LOGO Current time and date Tank real time temperatu Oven real time temperatur Shortcut program Waste gas concentration Concentration after filter of waste gas Status column Warnings Message column Station Message column Functions Button column System menu Select program operation running Log off Shut down ...

Страница 24: ...on window is displayed During program running buttons of progress and pause are available Click the progress button and the operator can review the residual running time of the current running program including the residual time of the current station and total residual time of this program Click the Pause button and the program will pause with the voice prompt Pausing In the meantime a prompt box...

Страница 25: ...rrent interface Click to save the current setting and exit Click to exit from the current menu the new item is saved when exiting Click to exit from the current menu and any modification that has been made currently is not saved Close the current dialog box Fig 17 Click the menu icon enter the system menu interface and click any of the icons to enter the next level editing and setting Fig 17 ...

Страница 26: ... types Alarm type 1 When user s intervention is needed alarm 1 is generated Alarm type 2 When an error occurs or user s intervention is urgently needed alarm 2 is generated Alarm type 3 When a fatal error occurs alarm 3 is generated Execute operations according to instructions provided in the error message In case of repeated occurrence of the same error please contact Department of Technical Serv...

Страница 27: ...nterface The interface provides parameter setting detection maintenance time setting and version query Fig 20 Fig 19 The alarm management function allows users to set whether to start voice alarm If the box is checked a voice prompt is played when the type of error occurs If the box is not checked no voice prompt is played when the type of error occurs Fig 20 ...

Страница 28: ...V1 0 20170825 28 5 Use Parameter settings Fig 21 ...

Страница 29: ...s the ventilation system to perform filtering when detecting that gas concentration is greater than 40 ppm so that gas concentration value is reduced Oven station temp Set the range from 0 70 touch and pull the prompt bar slide block to a proper value and then release Click the Setting button and the setting of oven temperature monitoring is completed When the oven is heated to the preset temperat...

Страница 30: ...the program When the device is running multiple times the robot is busy or the site time conflicts The new shelf can not be loaded and causes the dye to wait for a long time at the loading site At this point can be set to the first step of the procedure need to wait for the reagent the system default optional reagent for the dip for too long and no effect on the dye effect of reagents such as xyle...

Страница 31: ...shing stations to ensure that the water inlet functions at the W5 W6 W7 and W8 stations are good Drainage test Click the button Drainage test and the W5 W6 W7 and W8 stations start water inlet simultaneously to check for blocking The water inflow lasts 5 minutes Oven test Click the button Oven Test and the system starts the oven for heating to inspect whether the temperature rise is normal Fan tes...

Страница 32: ...ting is displayed Touch the screen to select hour minute and second successively and use the right arrowhead to press the buttons for setting After the setting is completed press the button of Save to exit Today Select a date month day and year or move the arrow on the calendar When an interface of other date is displayed click Today and the date automatically returns to the date interface of toda...

Страница 33: ...rn users about reagent changing and help users count the consumption of reagents Fig 24 Setup QC The quality control interface is divided into two areas The upper half area is the station selection column and the user can touch to select a reagent station and activate the lower half area of quality control to set reagent quality control at this station Fig 25 Fig 24 Fig 25 Station Selection Column...

Страница 34: ...f frame or number of days in the remaining columns After that click the button Save and the reagent warning function of the current station is activated Fig 26 Reagent management warning and instructions After the setting of the reagent stations that require quality control is completed as described above the top of the reagent stations on the quality control interface and main interface is indica...

Страница 35: ...layed in red When the function of warning based on the sequence of frame is enabled for the R1 station effective dip dyeing is set to 30 frames and others are set to 5 frames When the number of frames ranges from 0 to 24 the R1 station is displayed in green when the number ranges from 25 to 29 the color changes to yellow when the number is 30 and more the color is red Fig 27 Before quality control...

Страница 36: ...he information about recent reagent changes at all stations and setting of reagent use Fig 28 Query according to programs This interface records the information about the number of staining frames after the last reagent changes at all stations and setting of warnings Fig 29 Fig 28 Fig 29 ...

Страница 37: ...erying reagent change records reagent station changing time and operator After clicking any of the modes the user can click the query condition box below and select a query condition from the drop down box The results will be displayed at the bottom in the form of data list Fig 30 Fig 30 ...

Страница 38: ...ing programs of the apparatus After entering a value in the time item the user can click the corresponding button and perform query based on daily monthly or accumulative data The query results will be displayed at the bottom in the form of data list and chart Fig 31 Fig 31 Date Months Cumulative ...

Страница 39: ...rical running programs and reagent use details provide data export functions Users can click the button Export after query on the module interfaces according to search criteria The system s default export route is U Disc ROOT DIRECTORY Stainer Data Export File Fold Module Name File Fold The default file format is Excel and default picture format is PDF Exported files are named as Module Name Date ...

Страница 40: ...d executing operations of deleting and changing the permission of users Ordinary user has the ordinary use permission of the system can only check the current user s account information can only change the password of the current user and cannot execute the operations of deleting user and changing user permission In addition an ordinary user has no permission to set reagent change and time and cha...

Страница 41: ...ouch the input column to display the soft keyboard and input the job number The maximum length of a job number is 10 numeric characters Touch the right inverted triangle button to display a drop down option box and click to select a user type Touch the input column to display the soft keyboard and input the password The maximum length of a password is 10 numeric characters Re enter the confirmed p...

Страница 42: ...ty Touch to select any user from the user list Click the button Change Permission and the dialog box for changing user permission is displayed Click the right triangle button in the Type column to display the drop down permission option box and touch it to choose Click Confirm to complete the permission changing The respective number of both administrator user and ordinary user can be more than on...

Страница 43: ...T to enter the AAT parameter setting interface The user can touch to enable this function and set parameters It should be noted that this function is only applicable to hematoxylin and eosin The operator can only change the password of the current user that logs in to the system instead of the password of other user If the two input passwords are different an error message is displayed after Confi...

Страница 44: ...fth stand is used for hematoxylin dyeing the dyeing time increases by 2 seconds on the basis of program set time and so on Eosin setting After touching to activate the option of eosin the operator can perform setting based on the days of dyeing or number of stands Setting based on the days of dyeing For example if the frequency is set to every 2 days the dyeing time increases by 1 second This mean...

Страница 45: ...nd a numerical symbol of the station Station type parameters are as follows R Reagent station W Water washing station O Oven station L Loading station U Unloading station Fig 39 The system provides 26 stations Each station corresponds to a button and the operator can directly visit the Station Information menu by pressing the button Fig 40 ...

Страница 46: ... The changing of Unloading Station U24 U26 is not allowed The changing of Oven Station 013 is not allowed The changing of Water Washing Station W5 W9 into Reagent Station is allowed but the changing into Oven Station Loading Station and Unloading Station is not allowed The changing of the remaining reagent stations is not allowed Fig 41 By changing the loading and unloading stations you can set th...

Страница 47: ...xceeds the preset maximum number of slide stands or maximum use days the top of the station on this interface will be displayed in red prompting the user to change the reagent For the details please refer to Section 5 5 4 Please make sure that the reagent in the reagent tank is consistent with the name of the reagent configured on the reagent management interface otherwise damage of specimen or po...

Страница 48: ...ons below the table and the programs can be edited by operating the buttons When certain program on the program list interface is displayed in red and black and its effective status is no Fig 43 the program has at least one step line that is different from the reagent at the corresponding station Select the program and click the button View to view the program interface The abnormal step line is d...

Страница 49: ...gram list Before copy the operator must touch to select a program Click to delete a program in the list The operator must touch to select a program before deleting it Click to create a blank program The new program is located in the first line of the program list 5 7 1 Creation of Staining Program Press the button creation and the system creates an unnamed blank program list Input program name Cli...

Страница 50: ...complete the defining of program steps the following parameters must be input 1 The stations involved in the steps to be executed 2 The reagents that correspond to this step that must be allocated to the station 3 Step duration 1 second to 59 minutes and 59 seconds 4 Leaching time 0 to 30 seconds 5 Whether necessary to precisely define as yes no 6 Frequency of vibrating the tank 0 30 times If two ...

Страница 51: ...lect a proper station as the current step allocation station Reagent automatically added after station allocation the operator should allocate reagent to the station in advance otherwise the program cannot be created For details please refer to Section 5 6 2 Fig 47 Except the water washing station each station can only be selected once in the same program and selected stations are highlighted by g...

Страница 52: ...curate steps In the Whether accurate column complete selection by touching to choose yes or no the default setting is no Definition of tank vibrating frequency Touch the column of tank vibrating frequency to display the soft keyboard and enter a number to complete the input the default setting is 0 and the input value ranges from 0 to 30 Fig 48 Except unloading step time which can set by default d...

Страница 53: ...The user can press the bottom of the interface as needed to select the speed of the robotic arm The speed of the manipulator in the drop down box includes the slow lifting the medium speed and the three modes Figure 50 The system defaults to slow mention The maximum lateral speed of the manipulator is 1 m s the rise time is 0 4 seconds the rise time is 0 76 seconds and the quick pick up speed can ...

Страница 54: ...e system will automatically create a copied program named as Original Program Name_COPY The program is When a program is not named or the program running time and station are not specified a window displays an error message that prompts wrong step when the Save button is clicked Only after the operator confirms the message and makes modification according to the prompt can the program be saved The...

Страница 55: ...ogram management interface touch to select a program in the program list and click the View button The program view interface is displayed Modification and editing of columns of program step line Touch any program step line and click the View button below The step editing interface is displayed For the editing method refer to Section 5 7 1 Entire operation of program line By pressing the buttons I...

Страница 56: ... to select any program re click the triangle on the right side of Shortcut Key to display the drop down option box and click Confirm after touching and selection The selected program is then allocated to the shortcut keys of P1 P2 P3 and P4 After the program is allocated to the shortcut keys you can press the Clear button to activate it or press the Clear button to reset it on the P1 P2 P3 and P4 ...

Страница 57: ...through the loading station Open the drawer and insert slide stands at the loading station Close the drawer Pay attention to the operation sequence at the initial phase of oven station The filing of reagent is forbidden during heating or the heating operation is forbidden after reagent filling at the oven station The highest temperature of oven can be 70 Reagent may catch fire damaging the apparat...

Страница 58: ... loading station where the slide stands are located is displayed in blue to show that the station is prepared Quick Start Program For common programs the user can set shortcut programs by referring to Section 5 7 5 and click the shortcut program starting button on the user interface The slide stands of the loading station will execute the staining operation according to the allocated staining proc...

Страница 59: ...am be used by the departments dealing with a large number of staining samples and relatively simple staining processes Please set a proper starting time before enabling this function Do not execute other operations after the automatic starting function is used to recognize slide stands otherwise the system recalculates the time leading to prolonged automatic starting time If it is necessary to run...

Страница 60: ...ion remaining time of the station and total residual time of the program By clicking Details the user can enter the program step interface which displays information about all steps of the running program Completed program steps are displayed in gray and the program steps that are being executed are displayed in flickering green Only when the apparatus is in user mode and at least one staining pro...

Страница 61: ...ll directly enters the unloading station The staining program in discontinued status in the current progress list is displayed in red background This function is applicable to the program that requires rapid execution such as frozen dyeing The use of discontinuation is not recommended for some stations such as 1 hydrochloric alcohol solution Forced use may result in impairment of specimens On the ...

Страница 62: ... arm completes the program steps that are being executed After the mechanical arm is at the position of suspension the protective cover can be re started for other operations Only when the apparatus is in user mode and at least one staining program is running can the Pause button be used In other status the button is displayed in gray and is unavailable When staining is being executed or in the mo...

Страница 63: ...the program step 5 8 7 Completing staining procedure Unloading slide stands After completion of the program the slide stands are located in the unloading station the unloading station is displayed in red and a voice prompt is played prompting the user to take out the slide stands Please carefully open the drawer and take out the slide stand The icon of the station turns from red to gray The entire...

Страница 64: ...container under the temperature of more than 65 to avoid station deformation Inside of the Apparatus Mechanical arm Outer surface Touch screen Water rinsing container and reagent Wash the container after the reagent tank is taken out Use ordinary neutral detergent to clean the interior wall of the stainless steel and wash it with water The mechanical arm contains sensitive electronic elements Ther...

Страница 65: ...service personnel of Dakewe Drain exit tube Slide stand Oven Frequently inspect whether the wastewater pipe has accumulative filth especially waterweeds Wash them if necessary 5 sodium hypochlorite must be used to rinse the liquid discharging system in order to prevent bacterial growth However the metal parts shall be prevented from long time exposure to this solution Therefore a large amount of w...

Страница 66: ...on the operation can be continued Immediately contact Dakewe for technical support when the apparatus is not working 3 The mechanical arm experiences an error and cannot reset automatically System fault Shut down the apparatus power and reset the apparatus When the running staining program is influenced perform manual operations to complete unfinished steps Immediately contact Dakewe for technical...

Страница 67: ...start the water tank heating function Disabling reagent heating function does not affect other staining procedures Immediately contact Dakewe for technical support when the apparatus is not working 9 The oven is not started System hardware fault On the interface of menu setting parameter setting disable the oven heating functions and use other heating devices of the department Immediately contact ...

Страница 68: ... the mechanical arm jams the tank The reagent tank is not opened and some slide stands are not taken out from the reagent tank Open the reagent tank cover or take out the slide stands inside the station that experiences the tank jamming situation Inspect whether the slide stands are damaged and unhooked Click the button Reduce Mechanical Arm on this display screen after confirmation that there is ...

Страница 69: ...when the apparatus is not working 17 The staining program cannot run displayed in red The configuration of program station is different from that of the actual station Inspect the reagent configuration in the current station and update the staining program Immediately contact Dakewe for technical support Note After a fault occurs please remove the fault according to the temporary solutions listed ...

Страница 70: ...on the back of the apparatus Inspect the fault table in Section 7 2 and find the fuse to be replaced The fuse labeled as 3A can only be used to replace the fuse in F1 F3AL250V F1 3A F2 5A Before a fuse is changed maintain that the apparatus is shut down and the power line is unplugged Only the backup fuse provided together with the apparatus can be used to replace the damaged fuse Important The fo...

Страница 71: ...e fuse and fuse socket pop up automatically after the release Remove the damaged fuse 2 from the fuse socket 1 and insert the backup fuse of the correct type As indicated by Fig 54 press in the fuse socket installed with the replacing the fuse along the blue arrow direction and rotate the fuse socket by 1 4 circle along the red arrow direction The fuse socket will be jammed to complete the operati...

Страница 72: ...ew activated carbon filtration net Close the stamping parts around the filtration net along the negative direction of the red arrow as indicated in the second step and tightly lock the filtration net As indicated by the left figure insert the assembled filtration net modules into the installation site of filtration network along the blue arrow direction During the insertion of filtration net modul...

Страница 73: ... maintenance services please contact the local sales representatives or distributor that sells this apparatus Technical service information If it is necessary to require technical services or change parts please contact the local sales representative or distributor that sells this apparatus Please provide the following information Model name and sequential number of the apparatus Apparatus locatio...

Страница 74: ...ank Water washing reagent tank Slide stand Main power line Power jumper Modules of water inlet pipe Water outlet pipe Ventilating tube Order No 023 000136 00 022 000066 00 023 000137 00 023 000012 00 100 000003 00 100 000004 00 100 000004 00 026 000032 00 100 000005 00 ...

Страница 75: ...ng Shenzhen Biological Medicine Innovation Industry Base Jin Hui Road 14th Keng Zi Street Pingshan New District Shenzhen China Product record number YSXB No 20160143 Product license record number YSSYJXSCB No 20160031 Telephone number 0755 26413421 Fax 0755 27383156 Customer Service 400 6304366 Website http www dakewe com Company Name Lotus Global Co Ltd Company Address 1 Four Seasons Terrace West...

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