1-6
AX-4030
AUTION MAX AX-4030 OPERATING MANUAL
B
1.1.4
Measurement Principle
This instrument measures purpose-specific test strips by dual-wavelength reflectance measurement
(single wavelength for BLD) and urine specific gravity (S.G.) by reflection refractometry.
This section explains the principles for measuring “test strips”, “specific gravity”, “color”, and
“turbidity”.
Test Strip Measurement
Test strips loaded in the feeder are sent one at a time to the tray inside the instrument and carried
to the sample staining position by the introduction arm. At the sample staining position, the
nozzle that aspirated the sample from the sample tube in the sampler stains each reagent pad (each
measurement item) of the test strip. Once stained, the test strip is transported to the optical block.
Within about 60 sec of staining, the test strip reacts and changes color, therefore its reflectivity is
measured in that moment. When measurement has been completed, the test strip is discarded in the
waste box.
The measurement unit irradiates the reagent pad with light of 2 wavelengths from a 5-wavelength
LED, the reflected light of which is picked up by a single detector.* Moreover, by measuring the
reflectivity of the color correction pad of the test strip, destabilizing factors such as the amount of
reflected light and colored samples are corrected.
* The irradiated wavelength combinations differ according to measurement item.
Wavelengths by measurement item
Measurement item
GLU
PRO
BIL
URO
PH
BLD
KET
NIT
LEU
Measurement
wavelength (nm)
635
635
565
565
635
635
565
565
565
Reference
wavelength (nm)
760
760
760
760
760
–
760
760
760
Reflectivity is measured with the formula below.
R = (Tm • Cs) / (Ts • Cm)
R:
Reflectivity
Tm:
Amount of reflected light coming from reagent pad at measurement wavelength
Ts:
Amount of reflected light coming from reagent pad at reference wavelength
Cm:
Amount of reflected light coming from color correction pad at measurement
wavelength
Cs:
Amount of reflected light coming from color correction pad at reference
wavelength
However, BLD is measured with only one wavelength and calculated with the formula below.
R = Tm / Cm
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