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Conductivity sensor
The conductivity sensor, CE-101, is included with the flow kit (for all other sensors, the
flow kit includes only the flow cells). The conductivity sensor is located after the flow
meter cell in the flow path. It is primarily used to verify the conductivity of buffer solutions.
Conductivity measurement is temperature compensated and thus dependent on correct
temperature readings. The conductivity sensor cable is connected to the system cabinet
using a screw-on connector secured by a fastening nut. The standard setting of the cell
constant is 12.5 cm
-1
.
UV cell
The UV cell, AE-131, is located after the conductivity cell in the flow path. The cell consists
of a transparent rectangular flow cell. The cell is mounted in a holder containing a UV
LED emitting at 280 nm and two detectors measuring the UV absorbance of the liquid.
For correct measurements it is important that UV cell and sensor windows are properly
fitted, kept clean and dry. The temperature of the process liquid must not be lower than
10ºC from the ambient temperature. Otherwise there is a risk for condensation of
moisture on the flow cell window.
ÄKTA ready Operating Instructions 28960345 AD
51
3 System description
3.4 Equipment