The Elution block
Now that you have defined and saved the Fraction and Fraction_Stop blocks, proceed
by defining the elution block:
Action
Step
Create a block named
MyElution
and set its Base to Volume.
1
Insert an Inlet instruction at time 0.00. The instruction should be set to the
inlet used for the elution liquid, for example Inlet5 (the outlet is left unchanged
from previous block).
2
Create and insert a watch instruction, which starts fractionation when the
UV level is above 100 mAU. This is done by selecting instruction
Watch_UV_AT131 and, in the parameters area, setting the Test parameter
to Greater_Than and the Value parameter to 100 mAU. In the popup menu
under Action, select the Fraction block that you previously created.
3
Using the same procedure as in the previous step, create another watch
instruction for stopping fractionation when UV goes below 90 mAU. Set the
parameter Test to Less_Than and Value to 90 mAU, and select the previously
created Fraction_Stop block, under Action.
4
The MyElution block uses two watch in-
structions that trigger start and stop of
the fractionation phase. Using external
blocks like this is the recommended way
of defining fractionation in the Method
Editor.
ÄKTA ready Operating Instructions 28960345 AD
165
10 Creating a method
10.2 An example of using the Method Editor
10.2.9 Block 8: Elution
