10.2.8
Block 7: Washing unbound sample
After applying the sample you need to define instructions to wash out any unbound
sample from the column.
Action
Step
Create a block named
MyWashOutSample
, and set its Base to Time.
1
Select the inlet for the wash liquid by inserting an Inlet instruction at time
0.00. Set the inlet to, for instance, Inlet6.
2
Insert a Hold_Until instruction that is
triggered when UV readings are
Less_Than a set limit (e.g., 100 mAU).
At this point, the flow path is consid-
ered to be clean and the block will
finish.
3
The MyWashOutSample block first
switches to the inlet of the washing
liquid and then washes the flow path
until the UV readings indicate it is
clean.
ÄKTA ready Operating Instructions 28960345 AD
163
10 Creating a method
10.2 An example of using the Method Editor
10.2.8 Block 7: Washing unbound sample
