Set up for calibration
The set up for calibration procedure is run to ensure that no air bubbles are present in
the system tubings. A column should be connected to make the calibration conditions
similar to those of a real run.
Action
Step
Open the instructions box in the System Control module.
1
Set the flow path in the instructions box under FlowPath, Inlet, Outlet, Air-
Trap, Column.
2
Use an appropriate inlet and outlet, set AirTrap to Bypass and Column to
Bypass.
3
Connect the inlet tubing to appropriate buffer vessels.
4
Start pump A (%B = 0) with a ManFlow of 50%.
5
Run the pump until the flow meter signal is stable.
6
Start pump B (%B = 100) with a ManFlow of 50%.
7
Run the pump until the flow meter signal is stable.
8
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ÄKTA ready Operating Instructions 28960345 AD
7 ÄKTA ready gradient
7.3 Calibrate and operate the ÄKTA ready gradient pump
