Biotek Synergy H1, Руководство оператора

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Solution B
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Deionized water
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FD&C Yellow No. 5 dye powder (typically 90% pure)
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Tween 20 (polyoxyethylene (20) sorbitan monolaurate) or BioTek wetting agent (PN
7773002) (a 10% Tween solution)
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Precision balance with capacity of 100 g minimum and readability of 0.001 g
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Weigh boat
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1-liter volumetric flask
1. Weigh out 0.092 g of FD&C Yellow No. 5 dye powder into a weigh boat.
2. Rinse the contents into a 1-liter volumetric flask.
3. Add 0.5 mL of Tween 20, or 5 mL of BioTek’s wetting agent.
4. Fill to 1 liter with DI water; cap and shake well. The solution should measure
approximately 2.000 OD when using 200 μL in a flat-bottom microwell.
Test Procedure
i Be sure to use a new microplate. Debris, fingerprints, or scratches may cause
variations in readings.
1. Using freshly prepared stock solution (Solution A or B), prepare a 1:2 dilution using
deionized water (one part stock, one part deionized water; the resulting solution is a
1:2 dilution).
2. Pipette 200 μL/well of the stock solution into column 1.
3. Pipette 200 μL/well of the diluted solution into column 2.
4. Create a Gen5 experiment based on the Synergy H1 Abs Test 1 protocol and read
the plate. When prompted, rotate the plate 180 degrees and continue.
5. When the experiment is finished:
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Save the experiment. Refer to the instructions on page 80 to perform calculations
and determine pass/fail.
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Troubleshooting tips are provided on page 82 .
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Test descriptions are provided on page 77 .
Synergy H1 Operator's Manual
Absorbance Liquid Tests | 115