Biotek Synergy H1, Руководство оператора

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Product Description
The Synergy H1 is a hybrid multi-mode microplate reader. Depending on the model,
detection modes include fluorescence intensity (FI), fluorescence polarization (FP), time-
resolved fluorescence (TRF), luminescence, and UV-visible absorbance. The instrument is
modular, and upgrade options are available; contact BioTek Customer Care for more
information.
The reader is computer-controlled using Gen5 software for all operations, including data
reduction and analysis. The Synergy H1 is robot accessible and compatible with the
BioStack Microplate Stacker. Gen5 supports OLE automation to facilitate integration into an
automated system.
The Synergy H1 can perform reads using a filter cube or a monochromator*. The filter-
based system can perform fluorescence and luminescence reads. Filter fluorescence uses a
xenon flash light source, along with interference filters and dichroic mirrors for wavelength
specificity and a photomultiplier tube (PMT) detector. To run a fluorescence polarization
protocol, the filter cube must contain polarizing filters. Luminescence is measured through
an empty filter position in the filter cube; filters can be used if light filtering is necessary.
The monochromator-based system, which has both top and bottom probes, is used for
absorbance, fluorescence, and luminescence. Absorbance measurements are made using
the reader’s monochromator optics. The xenon lamp allows for both UV and visible light
measurements. The monochromator provides wavelength selection from 230–999 nm in 1-
nm increments. Available read methods are endpoint, area scan, spectral scanning, and
pathlength correction. For luminescence reads, the Synergy H1 has a direct-to-PMT
channel (no filtering, white light only). You can also use the monochromator optics for
luminescence spectral scanning.
The Synergy H1 has top and bottom incubation from 4°C over ambient to 45°C, controlled
via a software-adjustable gradient. Internal plate shaking, with both linear and orbital
modes, is supported to ensure that reagents are thoroughly mixed prior to reading. The
Synergy H1 supports the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with
128 x 86 mm geometry as well as the Take3 and Take3 Trio Micro-Volume Plate.
i Use of microplates other than those listed here can result in positioning errors during
program execution.
Models with injectors support dual-reagent dispensing to 6-, 12-, 24-, 48-, 96-, and 384-
well microplates. An external dispense module pumps fluid from the supply bottles to the
two injectors located inside the instrument. Models that support the gas controller can
control the CO
2
or O
2
concentrations in the reading chamber for CO
2
- or O
2
-sensitive
assays.
* This dual light path capability in the H1 MF model is protected by U.S. patent number
8,218,141.
2 | Chapter 1: Introduction
BioTek Instruments, Inc.