Biotek Synergy H1, Руководство оператора

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Test Procedure for Models without Absorbance Capability
If you are not using a BioTek absorbance reader for this procedure, prepare your reader
to perform two reads with the following characteristics:
80 µL Read 20 and 5 µL Read
Primary Wavelength: 405 nm 630 nm
Reference Wavelength: 750 nm 750 nm
Plate Columns: 1–4 5–12
1. Prime both dispensers with 4000 μL of deionized or distilled water.
2. Remove the inlet tubes from the supply bottles. Prime both dispensers with the
Volume set to 2000 μL. This prevents the water from diluting the dye.
3. Fill a beaker with at least 20 mL of the green dye solution. Prime both dispensers
with 2000 μL of the solution. When finished, remove the priming plate from the
carrier.
4. Create an experiment based on the Synergy H1 Disp 1 Test No Read protocol.
5. Place a new 96-well microplate on the balance and tare the balance.
6. Place the plate on the microplate carrier.
Running a dispense protocol with no plate on the carrier will
contaminate the reading chamber with spilled fluid.
When each dispense step is finished, you will weigh the plate, record the
weight, tare the balance with the plate on it, and then place the plate back
on the carrier for the next step.
7. Initiate a plate read. Gen5 will prompt you to empty the tip priming trough.
8. When ready, proceed with the experiment. The sequence is as follows:
l
80 μL/well is dispensed to columns 1–4.
l
When prompted, remove the plate and weigh it. Record the weight and tare the
balance. Place the plate on the carrier.
l
20 μL/well is dispensed to columns 5–8.
l
When prompted, remove the plate and weigh it. Record the weight and tare the
balance. Place the plate on the carrier.
l
5 μL/well is dispensed to columns 9–12.
l
When prompted, remove the plate and weigh it. Record the weight.
l
Manually pipette 150 μL of deionized or distilled water into all 12 columns, on top
Synergy H1 Operator's Manual
Injection System Tests | 137