Biotek Synergy H1, Руководство оператора

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Absorbance Liquid Test 2
The recommended method for testing the instrument's alignment, repeatability, and
accuracy is to use Absorbance Test Plate BTI #7260522 (see page
112 ). If the test plate
is not available, however, Liquid Test 2 can be used for these tests.
Materials
Manufacturer part numbers are subject to change.
l
New 96-well clear flat-bottom microplate (Corning Costar #3590 recommended)
l
Ten test tubes, numbered consecutively, set in a rack
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Calibrated hand pipette (Class A volumetric pipette recommended)
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Stock Solution A or B (see instructions for Liquid Test 1)
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0.05% solution of deionized water and Tween 20
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Gen5 protocol Synergy H1 Abs Test 2.prt described on page 79
Test Procedure
1. Create a percentage dilution series, beginning with 100% of the original concentrated
stock solution (A or B) in the first tube, 90% of the original solution in the second
tube, 80% in the third tube, all the way to 10% in the tenth tube. Dilute using the
0.05% solution of deionized water and Tween 20. This solution can also be made by
diluting the BioTek wetting agent 200:1.
Tube Number 1 2 3 4 5 6 7 8 9 10
Volume of original concentrated solution (mL) 20 18 16 14 12 10 8 6 4 2
Volume of 0.05% Tween solution (mL) 0 2 4 6 8 10 12 14 16 18
Absorbance expected if original solution is
2.000 OD at 200 µL 2.0 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2
The choice of dilutions and the absorbance of the original solution can be
varied. Use this table as a model for calculating the expected absorbances of a
series of dilutions, given a different absorbance of the original solution.
2. Pipette 200 μL of the concentrated solution from Tube 1 into each well of the first
column, A1 to H1, of a new flat-bottom microplate.
3. Pipette 200 μL from each of the remaining tubes into the wells of the corresponding
column of the microplate (Tube 2 into wells A2 to H2, Tube 3 into wells A3 to H3, and
so on).
4. Create a Gen5 experiment based on the Synergy H1 Abs Test 2 protocol and read
116 | Chapter 8: Instrument Qualification Procedures
BioTek Instruments, Inc.