Biotek Synergy H1, Руководство оператора

Страница: 149 / 190

Corners/Sensitivity/Linearity (FI) Tests:
The materials listed here are for use with Sodium Fluorescein.
Methylumbelliferone (MUB) may be used as an alternate or supplemental
method for conducting the FI tests for the Top optics; see page
130 .
If using test kit BTI #7160010 or #7160013 (see page 5 ) the buffer and SF are
pre-diluted.
l
Buffer:
o
NIST-traceable Sodium Borate Reference Standard (pH 9.18) (e.g., Fisher-
Scientific 1 L Sodium Borate Mfr. #159532, or equivalent),
or
o
Phosphate-Buffered Saline (PBS), pH 7.2–7.6 (e.g., Sigma tablets, Mfr.
#P4417, or equivalent) and pH meter or pH indicator strips with range 4–10
l
Sodium Fluorescein Powder (1-mg vial, BioTek PN 98155)
l
If testing both Top and Bottom optics (monochromator-based fluorescence only):
o
A new, clean 96-well glass-bottom Greiner SensoPlate (Mfr. #655892); or a
clean Hellma Quartz 96-well titration plate (Mfr. #730.009.QG); or equivalent
l
If testing only the Top optics:
o
A new, clean 96-well solid black microplate, such as Corning Costar #3915, or
equivalent
l
Excitation filter 485/20 nm installed
l
Emission filter 528/20 nm installed
l
510 nm dichroic mirror installed
Fluorescence Polarization (FP) Test:
The FP Test may be conducted using the same plate as for the Top
Corners/Sensitivity/Linearity (FI) Tests.
l
The recommended test solutions are available from Invitrogen Corporation in their
“FP One-Step Reference Kit” (#P3088) or BioTek (#7160014; see page
5 ). This kit
includes:
o
(Green) Polarization Reference Buffer, 15 mL
o
Green Low Polarization Reference, 4 mL
o
Green High Polarization Reference, 4 mL
o
The Invitrogen kit also includes two red polarization solutions, not used
l
A new, clean, 96-well solid black microplate, such as Corning Costar #3915. A
Greiner SensoPlate can also be used.
l
Excitation filter 485/20 nm installed
Synergy H1 Operator's Manual
Fluorescence Liquid Tests | 123