Biotek Synergy H1, Руководство оператора

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These instructions briefly describe how to create an experiment and then read a plate in
Gen5. See the Gen5 Help system for complete instructions.
1. In the Gen5 Task Manager, select the Experiments icon and click Create using an
existing protocol .
2. Select the desired protocol and click OK .
3. Select a plate in the menu tree and select Plate > Read Plate # or click the Read
New icon.
4. When the read is complete, measurement values appear in Gen5.
5. Select File > Save and give the experiment an identifying name.
Dispense Module Control
Applies only to models equipped with injectors
Gen5 is used to perform several dispense functions, such as initialize, dispense, prime, and
purge. The Prime and Purge functions are introduced here; refer to the Gen5 Help system
for additional information.
Prime
Before running an experiment with a Dispense step, prime the system with the fluid to
be used.
1. Place the priming plate on the carrier.
2. Fill the supply bottle with a sufficient volume of the fluid to be used for the prime
and the assay. Insert the appropriate inlet tube into the bottle.
3. Select System > Instrument Control > Synergy H1 and click the Prime tab.
4. Select the Dispenser number (1 or 2) associated with the supply bottle.
5. Enter the Volume to be used for the prime. The minimum recommended prime
volume is 2000 μL.
6. Select a prime Rate, in μL/second.
7. Click Prime to start the process. When finished, carefully remove the priming
plate from the carrier and empty it.
If the priming plate is empty, the prime volume was too low.
Purge
To conserve reagent, Gen5 provides the option to purge fluid from the system back into
the supply bottle.
1. Select System > Instrument Control > Synergy H1 and click the Prime tab.
2. Select the Dispenser number (1 or 2) associated with the supply bottle.
3. Enter the desired purge Volume in μL (e.g., 2000).
Synergy H1 Operator's Manual
Gen5 Software | 37