Biotek Synergy H1, Руководство оператора

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When each dispense step is finished, you will weigh the plate, record the
weight, tare the balance with the plate on it, and then place the plate back
on the carrier for the next step.
7. Initiate a plate read. Gen5 will prompt you to empty the tip priming trough.
8. When ready, proceed with the experiment. The sequence is as follows:
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80 μL/well is dispensed to columns 1–4.
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When prompted, remove the plate and weigh it. Record the weight and tare the
balance. Place the plate on the carrier.
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20 μL/well is dispensed to columns 5–8.
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When prompted, remove the plate and weigh it. Record the weight and tare the
balance. Place the plate on the carrier.
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5 μL/well is dispensed to columns 9–12.
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When prompted, remove the plate and weigh it. Record the weight.
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Manually pipette 150 μL of deionized or distilled water into all 12 columns, on top
of the green test dye solution.
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Place the plate on the carrier for the shake and read steps.
9. When the experiment is complete, save the file with an identifying name.
10. Remove the plate from the carrier and set it aside.
11. Repeat the procedure using the Synergy H1 Disp 2 Test protocol and a new
microplate.
12. When the tests are complete:
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Prime both dispensers with at least 5000 µL of deionized water to flush out the dye
solution.
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Refer to the instructions on page 105 to perform calculations and determine
pass/fail.
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Test descriptions are provided on page 102 .
136 | Chapter 8: Instrument Qualification Procedures
BioTek Instruments, Inc.