Biotek Synergy H1, Руководство оператора

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Code Description and possible remedy
4xxx PMT overload well error at <well #xxx>
This error typically means that the fluid in a well has oversaturated
the PMT (i.e., the well is too bright). Try lowering the gain in the
read step.
To identify the well:
Wells are counted starting at A1, moving left-to-right, row-by-row.
The row and column of the well can be extracted from the well
number code by applying the following formula (example uses 8 x
12 geometry, 96-well plate):
1. Convert the ASCII hex string to a decimal equivalent. Ex: “057”
indicates 57 hex, yielding a well code of 87 decimal.
2. Row = (well code) / (columns in plate), rounded up to a whole
number. Ex: 87/12 = 7.25, indicating row 8 (or H).
3. Column = (well code) - ((row-1) * (columns in plate)). Ex: 87 -
((8 - 1) * 12) = column 3.
NOTE: If this code is returned during an area scan, it indicates the
scan point corresponding to the row/column equivalent in the
currently defined scan map, NOT the actual well where the error
occurred.
4Exy Detector saturated (too much light). Relative Fluorescing Units
(RFU) reached (99999).
x=0, 1; y=0–6
This error can indicate one of several scenarios. It is possibly due
to incorrect chemistry, e.g., the fluorescence standards dispensed
to the plate exceed expectations.
Try lowering the gain/sensitivity in your Read step(s).
For models with the dispense module, the internal chamber may
require cleaning (contact BioTek TAC).
If a 4E18 error is detected during monochromator-based
fluorescence, the luminescence probe may be picking up stray
light. Try installing a plug in the filter cube. Restart the reader.
148 | Appendix B: Error Codes
BioTek Instruments, Inc.