Biotek Synergy H1, Руководство оператора

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the Allowed Deviation for well A1. Since the Standard Deviation for well A1 is less
than 0.013, the well meets the test criteria.
3. Calculate results for Linearity:
o
For each of the three test solutions, calculate the average Mean OD for the
wells containing that solution (mean of wells A1 to H2, A3 to H4, and A5 to H6).
o
Perform a regression analysis on the data to determine if there is adequate
linearity. The three average Mean OD values are the “Y” values. The solution
concentrations are the “X” values (1.00, 0.75, 0.50). Since it is somewhat
difficult to achieve high pipetting accuracy when conducting linear dilutions, an
R
2
value of at least 0.9900 is considered adequate.
Troubleshooting
If an absorbance liquid test fails, try the following. If a test continues to fail, contact
BioTek TAC.
l
Check the microwells and plate carrier for debris that may have shifted and caused
changes.
l
Ensure the microplate is properly seated in the carrier.
l
As applicable, confirm that the plate was properly oriented in the "Normal" and
"Turnaround" positions.
l
Liquid Test 1 can fail due to the meniscus effect, which can cause readings to
decrease over time. If you suspect this may be the case, include a shake step
between the read steps in the protocol.
82 | Chapter 7: Instrument Qualification Process
BioTek Instruments, Inc.