Biotek Synergy H1, Руководство оператора

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recalibration, contact BioTek to schedule service.
l
Check the microplate carrier to ensure it is clear of debris.
Alignment Test
l
Ensure that the test plate is properly seated in the microplate carrier.
l
Check the four alignment holes (A1, A12, H1, H12) to ensure they are clear of debris.
l
Check the microplate carrier to ensure it is clear of debris.
Accuracy Test
l
Check the neutral-density filters to ensure they clean (positions C1, D4, E2, F5, G3,
H6). If needed, clean the filters with lens paper. Do not remove any filters, and do
not use alcohol or other cleaning agents.
l
Verify that the wavelength/expected OD values entered for the plate in Gen5 match
the information provided on the test plate's data sheet.
l
Check the calibration due date on the test plate's label. If the test plate is overdue for
recalibration, contact BioTek to schedule service.
Repeatability Test
l
Check the neutral-density filters to ensure there is no debris that may have shifted
between readings and caused changes.
l
Check the microplate carrier to ensure it is clear of debris.
Absorbance Liquid Tests
BioTek Instruments, Inc. has developed a series of liquid test procedures for testing your
reader's absorbance system.
Test Methods
Absorbance Liquid Test 1 confirms repeatability and alignment of the reader when a
solution is used in the microplate. If these tests pass, then the lens placement and
optical system cleanliness are proven. For the Repeatability portion of this test, two
columns containing a color-absorbing solution are read five times at 405 nm. For each
well, an "allowed deviation" is determined based on its Mean OD and the reader's
repeatability specification. Each well's Standard Deviation must be less than its Allowed
Deviation to pass. To confirm the reader’s mechanical alignment, the plate is rotated
180 degrees in the carrier (e.g., A1 is now in the H12 position) and the same two
columns are read. The initial and new OD readings are compared, using the reader's
accuracy specification. If the two readings in the same well do not meet specification,
the reader may be out of alignment.
Synergy H1 Operator's Manual
Absorbance Testing | 77