Bio-Tek ELx800, Руководство оператора

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Liquid Testing
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117
Stock Solution Formulation
The stock solution for Liquid Tests No. 1 and No. 2 may be formulated
from the ingredients listed below (Solution A), or by diluting a dye solution
available from Bio-Tek (Solution B).
Solution A
Required Materials:
• Deionized (DI) water
•
FD&C Yellow No. 5 dye powder (typically 90% pure)
•
Tween
®
20 (polyoxyethylene (20) sorbitan monolaurate) or
Bio-Tek wetting agent, PN 7773002
• Precision balance with readability of 0.001 g
• Weigh boat
• 1-liter volumetric flask
Preparation of Stock Solution:
1 Weigh out 0.092 gram of FD&C No. 5 yellow dye powder into a
weigh boat.
2 Rinse the contents into a 1-liter volumetric flask.
3 Add 0.5 ml of Tween 20, or 5 ml of Bio-Tek’s wetting agent.
4 Make up to 1 liter with DI water, cap, and shake well.
This should create a solution with an absorbance of about 2.000
when using 200 µl in a flat-bottom microwell. The OD value will
be proportional to the volume in the well and the amount of
FD&C No. 5 dye used. You can use a larger or smaller well
volume, or add more dye or water to adjust the solution. Note
that too small a well volume may result in increased pipetting-
related errors.
ELx800 Operator's Manual