Bio-Tek ELx800, Руководство оператора

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Chapter 4: Performance Verification and IQ/OQ/PQ Procedures
Alignment Test
1 Using the prepared plate, conduct a turnaround test by reading
the plate with the A1 well in the H12 position five times. This
test results in values for the four corner wells that can be used to
determine alignment.
2 Calculate the means of wells A1 and H1 in the normal plate
position (data is from Linearity Test) and in the turnaround
position (from Step 1 above). Compare the mean reading for
well A1 to its mean reading when in the H12 position. Next,
compare the mean values for the H1 well to the same well in the
A12 position. The difference in the values for any two
corresponding wells should be within the accuracy specification
for the instrument.
For example:
If the mean of well A1 in the normal position is 1.902, where the
specified accuracy is ± 1% ± 0.010 OD, then the expected range
for the mean of the same well in the H12 position is 1.873 to
1.931 OD.
(1.902 * 1% = 0.019 + 0.010 = 0.029, which is added and
subtracted from 1.902 for the range.)
3 If the four corner wells are within the accuracy range, the reader
is in alignment.
Accuracy Specifications:
For comparison in this test, the following accuracy specification
is applied, using Normal Mode and a 96-well microplate:
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm
Bio-Tek Instruments, Inc.