Bio-Tek ELx800, Руководство оператора

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Liquid Testing
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Procedure
1.
Check the absorbance of a sample of buffer solution A or B at
340 nm on the microplate reader. This solution, which will be
referred to as the
100% Test Solution , will have an optical
density (absorbance) of approximately 0.700 to 1.000. This value is
not critical, but it should be within this absorbance range.
If low, adjust up by adding
D
-NADH powder until the solution is at
least at the lower end of this range. Do not adjust if slightly high.
2. Carefully prepare a 75% Test Solution by diluting 15 ml of the
100% Test Solution:
If using the Sigma PBS Solution, use 5 ml as the diluent.
If using the 10x Concentrate PBS Solution, mix one part of the
concentrate with nine parts of DI water. Use 5 ml of this solution
as the diluent.
3. Carefully prepare a 50% Test Solution by diluting 10 ml of the
100% Test Solution:
If using the Sigma PBS Solution, use 10 ml as the diluent.
If using the 10x Concentrate PBS Solution, mix one part of the
concentrate with nine parts of DI water. Use 10 ml of this solution
as the diluent.
4. Pipette the three solutions into a new 96-well microplate:
150 µl of the 100% Test Solution into all wells of columns 1 and 2
150 µl of the 75% Test Solution into all wells of columns 3 and 4
150 µl of the 50% Test Solution into all wells of column 5 and 6
5. Read the microplate five times using Normal Mode, single
wavelength at 340 nm, no blanking (or blank on air).
6. Print the five sets of raw data or export it to an Excel spreadsheet
using KCjunior™ or KC4™.
If you are exporting the data to an Excel spreadsheet, perform
the calculations described on the following page, and keep the
spreadsheet for future tests.
ELx800 Operator's Manual